| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-163 |
Sentence |
denotes |
Manipulation of hyaluronan synthase expression in prostate adenocarcinoma cells alters pericellular matrix retention and adhesion to bone marrow endothelial cells. |
| T2 |
164-342 |
Sentence |
denotes |
Prostate cancer metastasis to bone marrow involves initial adhesion of tumor cells to the bone marrow endothelium, followed by transmigration and proliferation within the marrow. |
| T3 |
343-597 |
Sentence |
denotes |
Rapid, specific adhesion of highly metastatic prostate adenocarcinoma cells (PC3M-LN4) to bone marrow endothelial cell (BMEC) lines requires a pericellular hyaluronan (HA) matrix and correlates with dramatically up-regulated HA synthase (HAS) expression. |
| T4 |
598-728 |
Sentence |
denotes |
Non-metastatic prostate tumor cells (LNCaP) do not assemble a HA matrix, adhere poorly to BMECs, and express normal levels of HAS. |
| T5 |
729-845 |
Sentence |
denotes |
Preferential bone metastasis of prostate carcinoma cells may therefore be facilitated by tumor cell HA biosynthesis. |
| T6 |
846-989 |
Sentence |
denotes |
In this report, HAS gene expression was manipulated to investigate the direct impact of prostate tumor cell HA production on adhesion to BMECs. |
| T7 |
990-1090 |
Sentence |
denotes |
PC3M-LN4 cells stably transfected with antisense HAS2 and HAS3 failed to form pericellular matrices. |
| T8 |
1091-1215 |
Sentence |
denotes |
Adhesion of these transfectants to BMECs was significantly diminished, comparable to the low level exhibited by LNCaP cells. |
| T9 |
1216-1340 |
Sentence |
denotes |
Upon transfection with full-length HAS2 or HAS3, the non-adherent LNCaP cells retained pericellular HA and adhered to BMECs. |
| T10 |
1341-1496 |
Sentence |
denotes |
The results of this study are consistent with a model in which HA matrix formation, BMEC adhesion, and metastatic potential are mediated by HAS expression. |
