> top > projects > sentences > docs > PubMed:1168193 > annotations
sentences Home  

PubMed:1168193 JSONTXT 53 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-181 Sentence denotes Deficient uridine diphosphate-N-acetylglucosamine:glycoprotein N-acetylglucosaminyltransferase activity in a clone of Chinese hamster ovary cells with altered surface glycoproteins.
T1 0-181 Sentence denotes Deficient uridine diphosphate-N-acetylglucosamine:glycoprotein N-acetylglucosaminyltransferase activity in a clone of Chinese hamster ovary cells with altered surface glycoproteins.
T2 182-456 Sentence denotes We have reported the isolation of a clone (termed 15B) of Chinese hamster ovary (CHO) cells which are deficient in certain plant lectin-binding sites and have decreased amounts of sialic acid, galactose, and N-acetylglucosamine in its membranes (Gottlieb et al. (1974) Proc.
T2 182-456 Sentence denotes We have reported the isolation of a clone (termed 15B) of Chinese hamster ovary (CHO) cells which are deficient in certain plant lectin-binding sites and have decreased amounts of sialic acid, galactose, and N-acetylglucosamine in its membranes (Gottlieb et al. (1974) Proc.
T3 457-462 Sentence denotes Natl.
T3 457-462 Sentence denotes Natl.
T4 463-468 Sentence denotes Acad.
T4 463-468 Sentence denotes Acad.
T5 469-473 Sentence denotes Sci.
T5 469-473 Sentence denotes Sci.
T6 474-480 Sentence denotes U.S.A.
T6 474-480 Sentence denotes U.S.A.
T7 481-496 Sentence denotes 71, 1078-1082).
T7 481-496 Sentence denotes 71, 1078-1082).
T8 497-760 Sentence denotes This study demonstrates that extracts of 15B cells, in contrast to the parent cell line, do not transfer N-acetylglucosamine residues from UDP-GlcNAc to certain glycopeptide and glycoprotein acceptors containing terminal nonreducing alpha-linked mannose residues.
T8 497-760 Sentence denotes This study demonstrates that extracts of 15B cells, in contrast to the parent cell line, do not transfer N-acetylglucosamine residues from UDP-GlcNAc to certain glycopeptide and glycoprotein acceptors containing terminal nonreducing alpha-linked mannose residues.
T9 761-971 Sentence denotes The decreased enzyme activity could not be accounted for by the presence of inhibitors, altered pH, or Mn2+ requirements of the glycosyltransferase or increased N-acetylglucosaminidase activity in the extracts.
T9 761-971 Sentence denotes The decreased enzyme activity could not be accounted for by the presence of inhibitors, altered pH, or Mn2+ requirements of the glycosyltransferase or increased N-acetylglucosaminidase activity in the extracts.
T10 972-1270 Sentence denotes The finding that the 15B cell extracts have significant but reduced N-acetylglucosaminyltransferase activity toward a degraded orosomucoid acceptor suggests that these cells have a selective loss of one of several specific N-acetylglucosaminyltransferases which are present in the parent CHO cells.
T10 972-1270 Sentence denotes The finding that the 15B cell extracts have significant but reduced N-acetylglucosaminyltransferase activity toward a degraded orosomucoid acceptor suggests that these cells have a selective loss of one of several specific N-acetylglucosaminyltransferases which are present in the parent CHO cells.
T11 1271-1363 Sentence denotes The sialyl- and galactosyltransferase activities of 15B and parent CHO cells are comparable.
T11 1271-1363 Sentence denotes The sialyl- and galactosyltransferase activities of 15B and parent CHO cells are comparable.
T12 1364-1463 Sentence denotes Parent CHO and 15B cells were grown in radioactive glucosamine to label the membrane glycoproteins.
T12 1364-1463 Sentence denotes Parent CHO and 15B cells were grown in radioactive glucosamine to label the membrane glycoproteins.
T13 1464-1865 Sentence denotes Solubilization of these glycoproteins and passage over a Ricinus communis agglutinin I (RCA I) Sepharose affinity column revealed that no labeled 15B glycoprotein material bound, whereas 50 percent of the CHO membrane glycoproteins bound and could be eluted with the haptene lactose, demonstrating that 15B cells are virtually devoid of membrane oligosacharides capable of binding to the RCA I lectin.
T13 1464-1865 Sentence denotes Solubilization of these glycoproteins and passage over a Ricinus communis agglutinin I (RCA I) Sepharose affinity column revealed that no labeled 15B glycoprotein material bound, whereas 50 percent of the CHO membrane glycoproteins bound and could be eluted with the haptene lactose, demonstrating that 15B cells are virtually devoid of membrane oligosacharides capable of binding to the RCA I lectin.
T14 1866-2039 Sentence denotes The 15B membrane glycoproteins exhibited a marked shift toward glycoprotein species of lower molecular weight when examined by gel electrophoresis in sodium dodecyl sulfate.
T14 1866-2039 Sentence denotes The 15B membrane glycoproteins exhibited a marked shift toward glycoprotein species of lower molecular weight when examined by gel electrophoresis in sodium dodecyl sulfate.
T15 2040-2242 Sentence denotes It is proposed that this shift in the mobility of the 15B membrane glycoproteins results from a decreased glycosylation of a number of membrane glycoproteins relative to their counterparts in CHO cells.
T15 2040-2242 Sentence denotes It is proposed that this shift in the mobility of the 15B membrane glycoproteins results from a decreased glycosylation of a number of membrane glycoproteins relative to their counterparts in CHO cells.
T16 2243-2394 Sentence denotes The deficient N-acetylglucosaminyltransferase activity in 15B cells can account for the decreased glycosylation of the 15B cell membrane glycoproteins.
T16 2243-2394 Sentence denotes The deficient N-acetylglucosaminyltransferase activity in 15B cells can account for the decreased glycosylation of the 15B cell membrane glycoproteins.