| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-120 |
Sentence |
denotes |
IGF-I receptor-induced cell-cell adhesion of MCF-7 breast cancer cells requires the expression of junction protein ZO-1. |
| T2 |
121-299 |
Sentence |
denotes |
Hyperactivation of the insulin-like growth factor I receptor (IGF-IR) contributes to primary breast cancer development, but the role of the IGF-IR in tumor metastasis is unclear. |
| T3 |
300-441 |
Sentence |
denotes |
Here we studied the effects of the IGF-IR on intercellular connections mediated by the major epithelial adhesion protein, E-cadherin (E-cad). |
| T4 |
442-598 |
Sentence |
denotes |
We found that IGF-IR overexpression markedly stimulated aggregation in E-cad-positive MCF-7 breast cancer cells, but not in E-cad-negative MDA-MB-231 cells. |
| T5 |
599-720 |
Sentence |
denotes |
However, when the IGF-IR and E-cad were co-expressed in MDA-MB-231 cells, cell-cell adhesion was substantially increased. |
| T6 |
721-963 |
Sentence |
denotes |
The IGF-IR-dependent cell-cell adhesion of MCF-7 cells was not related to altered expression of E-cad or alpha-, beta-, or gamma-catenins but coincided with the up-regulation of another element of the E-cad complex, zonula occludens-1 (ZO-1). |
| T7 |
964-1098 |
Sentence |
denotes |
ZO-1 expression (mRNA and protein) was induced by IGF-I and was blocked in MCF-7 cells with a tyrosine kinase-defective IGF-IR mutant. |
| T8 |
1099-1194 |
Sentence |
denotes |
By co-immunoprecipitation, we found that ZO-1 associates with the E-cad complex and the IGF-IR. |
| T9 |
1195-1430 |
Sentence |
denotes |
High levels of ZO-1 coincided with an increased IGF-IR/alpha-catenin/ZO-1-binding and improved ZO-1/actin association, whereas down-regulation of ZO-1 by the expression of an anti-ZO-1 RNA inhibited IGF-IR-dependent cell-cell adhesion. |
| T10 |
1431-1672 |
Sentence |
denotes |
The results suggested that one of the mechanisms by which the activated IGF-IR regulates E-cad-mediated cell-cell adhesion is overexpression of ZO-1 and the resulting stronger connections between the E-cad complex and the actin cytoskeleton. |
| T11 |
1673-1764 |
Sentence |
denotes |
We hypothesize that in E-cad-positive cells, the IGF-IR may produce antimetastatic effects. |
