| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-65 |
Sentence |
denotes |
Molecular cloning and expression of a human chondroitin synthase. |
| T2 |
66-387 |
Sentence |
denotes |
We have identified a human chondroitin synthase from the HUGE (human unidentified gene-encoded large proteins) protein data base by screening with two keywords: "one transmembrane domain" and "galactosyltransferase family." The identified protein consists of 802 amino acids with a type II transmembrane protein topology. |
| T3 |
388-570 |
Sentence |
denotes |
The protein showed weak homology to the beta1,3-galactosyltransferase family on the amino-terminal side and to the beta1,4-galactosyltransferase family on the carboxyl-terminal side. |
| T4 |
571-839 |
Sentence |
denotes |
The expression of a soluble recombinant form of the protein in COS-1 cells produced an active enzyme, which transferred not only the glucuronic acid (GlcUA) from UDP-[(14)C]GlcUA but also N-acetylgalactosamine (GalNAc) from UDP-[(3)H]GalNAc to the polymer chondroitin. |
| T5 |
840-1013 |
Sentence |
denotes |
Identification of the reaction products demonstrated that the enzyme was chondroitin synthase, with both beta1,3-GlcUA transferase and beta1,4-GalNAc transferase activities. |
| T6 |
1014-1129 |
Sentence |
denotes |
The coding region of the chondroitin synthase was divided into three discrete exons and localized to chromosome 15. |
| T7 |
1130-1285 |
Sentence |
denotes |
Northern blot analysis revealed that the chondroitin synthase gene exhibited ubiquitous but markedly differential expression in the human tissues examined. |
| T8 |
1286-1484 |
Sentence |
denotes |
Thus, we demonstrated that analogous to human heparan sulfate polymerases, the single polypeptide chondroitin synthase possesses two glycosyltransferase activities required for chain polymerization. |
