| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-109 |
Sentence |
denotes |
Complete analysis of the glycosylation and disulfide bond pattern of human beta-hexosaminidase B by MALDI-MS. |
| T1 |
0-109 |
Sentence |
denotes |
Complete analysis of the glycosylation and disulfide bond pattern of human beta-hexosaminidase B by MALDI-MS. |
| TextSentencer_T2 |
110-224 |
Sentence |
denotes |
beta-hexosaminidase B is an enzyme that is involved in the degradation of glycolipids and glycans in the lysosome. |
| T2 |
110-224 |
Sentence |
denotes |
beta-hexosaminidase B is an enzyme that is involved in the degradation of glycolipids and glycans in the lysosome. |
| TextSentencer_T3 |
225-349 |
Sentence |
denotes |
Mutation in the HEXB gene lead to Sandhoff disease, a glycolipid storage disorder characterized by severe neurodegeneration. |
| T3 |
225-349 |
Sentence |
denotes |
Mutation in the HEXB gene lead to Sandhoff disease, a glycolipid storage disorder characterized by severe neurodegeneration. |
| TextSentencer_T4 |
350-416 |
Sentence |
denotes |
So far, little structural information on the protein is available. |
| T4 |
350-416 |
Sentence |
denotes |
So far, little structural information on the protein is available. |
| TextSentencer_T5 |
417-522 |
Sentence |
denotes |
Here, the complete analysis of the disulfide bond pattern of the protein is described for the first time. |
| T5 |
417-522 |
Sentence |
denotes |
Here, the complete analysis of the disulfide bond pattern of the protein is described for the first time. |
| TextSentencer_T6 |
523-663 |
Sentence |
denotes |
Additionally, the structures of the N-glycans are analyzed for the native human protein and for recombinant protein expressed in SF21 cells. |
| T6 |
523-663 |
Sentence |
denotes |
Additionally, the structures of the N-glycans are analyzed for the native human protein and for recombinant protein expressed in SF21 cells. |
| TextSentencer_T7 |
664-808 |
Sentence |
denotes |
For the analysis of the disulfide bond structure, the protein was proteolytically digested and the resulting peptides were analyzed by MALDI-MS. |
| T7 |
664-808 |
Sentence |
denotes |
For the analysis of the disulfide bond structure, the protein was proteolytically digested and the resulting peptides were analyzed by MALDI-MS. |
| TextSentencer_T8 |
809-913 |
Sentence |
denotes |
The analysis revealed three disulfide bonds (C91-C137; C309-C360; C534-C551) and a free cysteine (C487). |
| T8 |
809-913 |
Sentence |
denotes |
The analysis revealed three disulfide bonds (C91-C137; C309-C360; C534-C551) and a free cysteine (C487). |
| TextSentencer_T9 |
914-1117 |
Sentence |
denotes |
The analysis of the N-glycosylation was performed by tryptic digestion of the protein, isolation of glycopeptides by lectin chromatography and mass measurement before and after enzymatic deglycosylation. |
| T9 |
914-1117 |
Sentence |
denotes |
The analysis of the N-glycosylation was performed by tryptic digestion of the protein, isolation of glycopeptides by lectin chromatography and mass measurement before and after enzymatic deglycosylation. |
| TextSentencer_T10 |
1118-1231 |
Sentence |
denotes |
Carbohydrate structures were calculated from the mass difference between glycosylated and deglycosylated peptide. |
| T10 |
1118-1231 |
Sentence |
denotes |
Carbohydrate structures were calculated from the mass difference between glycosylated and deglycosylated peptide. |
| TextSentencer_T11 |
1232-1407 |
Sentence |
denotes |
For beta-hexosaminidase B from human placenta, four N-glycans were identified and analyzed, whereas the recombinant protein expressed in SF21 cells carried only three glycans. |
| T11 |
1232-1407 |
Sentence |
denotes |
For beta-hexosaminidase B from human placenta, four N-glycans were identified and analyzed, whereas the recombinant protein expressed in SF21 cells carried only three glycans. |
| TextSentencer_T12 |
1408-1540 |
Sentence |
denotes |
In both cases the glycosylation belongs to the mannose-core- or high-mannose-type, and some carbohydrate structures are fucosylated. |
| T12 |
1408-1540 |
Sentence |
denotes |
In both cases the glycosylation belongs to the mannose-core- or high-mannose-type, and some carbohydrate structures are fucosylated. |