| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-155 |
Sentence |
denotes |
Molecular cloning and characterization of a novel human galactose 3-O-sulfotransferase that transfers sulfate to gal beta 1-->3galNAc residue in O-glycans. |
| T2 |
156-356 |
Sentence |
denotes |
We have identified a novel galactose 3-O-sulfotransferase, termed Gal3ST-4, by analysis of an expression sequence tag using the amino acid sequence of human cerebroside 3'-sulfotransferase (Gal3ST-1). |
| T3 |
357-490 |
Sentence |
denotes |
The isolated cDNA contains a single open reading frame coding for a protein of 486 amino acids with a type II transmembrane topology. |
| T4 |
491-712 |
Sentence |
denotes |
The amino acid sequence of Gal3ST-4 revealed 33%, 39%, and 30% identity to human Gal3ST-1, Gal beta 1-->3/4GlcNAc:-->3'-sulfotransferase (Gal3ST-2) and Gal beta 1-->4GlcNAc:-->3'-sulfotransferase (Gal3ST-3), respectively. |
| T5 |
713-802 |
Sentence |
denotes |
The Gal3ST-4 gene comprised at least four exons and was located on human chromosome 7q22. |
| T6 |
803-982 |
Sentence |
denotes |
Expression of Gal3ST-4 in COS-7 cells produced a sulfotransferase activity that catalyzes the transfer of [(35)S]sulfate to the C-3' position of Gal beta 1-->3GalNAc alpha 1-O-Bn. |
| T7 |
983-1147 |
Sentence |
denotes |
Gal3ST-4 recognizes Gal beta 1-->3GalNAc and Gal beta 1-->3(GlcNAc beta 1-->6)GalNAc as good substrates, but not Gal beta 1-->3GalNAc(OH) or Gal beta 1-->3/4GlcNAc. |
| T8 |
1148-1356 |
Sentence |
denotes |
Asialofetuin is also a good substrate, and the sulfation was found exclusively in O-linked glycans that consist of the Gal beta 1-->3GalNAc moiety, suggesting that the enzyme is specific for O-linked glycans. |
| T9 |
1357-1471 |
Sentence |
denotes |
Northern blot analysis revealed that 2.5-kilobase mRNA for the enzyme is expressed extensively in various tissues. |
| T10 |
1472-1701 |
Sentence |
denotes |
These results suggest that Gal3ST-4 is the fourth member of a Gal:-->3-sulfotransferase family and that the four members, Gal3ST-1, Gal3ST-2, Gal3ST-3, and Gal3ST-4, are responsible for sulfation of different acceptor substrates. |
