| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-143 |
Sentence |
denotes |
Characterization of binding properties of urinary trypsin inhibitor to cell-associated binding sites on human chondrosarcoma cell line HCS-2/8. |
| T2 |
144-383 |
Sentence |
denotes |
Urinary trypsin inhibitor (UTI) forms membrane complexes with UTI-binding proteins (UTI-BPs) and initiates modulation of urokinase-type plasminogen activator (uPA) expression, which results in UTI-mediated suppression of cell invasiveness. |
| T3 |
384-731 |
Sentence |
denotes |
It has been established that suppression of uPA expression and invasiveness by UTI is mediated through inhibition of protein kinase C-dependent signaling pathways and that human chondrosarcoma cell line HCS-2/8 expresses two types of UTI-BPs; a 40-kDa UTI-BP (UTI-BP(40)), which is identical to link protein (LP), and a 45-kDa UTI-BP (UTI-BP(45)). |
| T4 |
732-810 |
Sentence |
denotes |
Here we characterize binding properties of UTI-BPs.UTI complexes in the cells. |
| T5 |
811-957 |
Sentence |
denotes |
In vitro ligand blot, cell binding and competition assays, and Scatchard analyses demonstrate that both UTI-BP(40) and UTI-BP(45) bind (125)I-UTI. |
| T6 |
958-1086 |
Sentence |
denotes |
A deglycosylated form of UTI (NG-UTI), from which the chondroitin-sulfate side chain has been removed, binds only to UTI-BP(40). |
| T7 |
1087-1306 |
Sentence |
denotes |
Additional experiments, using various reagents to block binding of (125)I-UTI and NG-UTI to the UTI-BP(40) and UTI-BP(45) confirm that the chondroitin sulfate side chain of UTI is required for its binding to UTI-BP(45). |
| T8 |
1307-1497 |
Sentence |
denotes |
Analysis of binding of (125)I-UTI and NG-UTI to the cells suggests that low affinity binding sites are the UTI-BP(40) (which can bind NG-UTI), and the high affinity sites are the UTI-BP(45). |
| T9 |
1498-1682 |
Sentence |
denotes |
In addition, UTI-induced suppression of phorbol ester stimulated up-regulation of uPA is inhibited by reagents that were shown to prevent binding of UTI to the 40- and 45-kDa proteins. |
| T10 |
1683-1767 |
Sentence |
denotes |
We conclude that UTI must bind to both of the UTI-BPs to suppress uPA up-regulation. |
