| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-170 |
Sentence |
denotes |
A pancreatic beta -cell-specific enhancer in the human PDX-1 gene is regulated by hepatocyte nuclear factor 3beta (HNF-3beta ), HNF-1alpha, and SPs transcription factors. |
| T2 |
171-243 |
Sentence |
denotes |
The PDX-1 transcription factor plays a key role in pancreas development. |
| T3 |
244-351 |
Sentence |
denotes |
Although expressed in all cells at the early stages, in the adult it is mainly restricted to the beta-cell. |
| T4 |
352-618 |
Sentence |
denotes |
To characterize the regulatory elements and potential transcription factors necessary for human PDX-1 gene expression in beta-cells, we constructed a series of 5' and 3' deletion fragments of the 5'-flanking region of the gene, fused to the luciferase reporter gene. |
| T5 |
619-794 |
Sentence |
denotes |
In this report, we identify by transient transfections in beta- and non-beta-cells a novel beta-cell-specific distal enhancer element located between -3.7 and -3.45 kilobases. |
| T6 |
795-980 |
Sentence |
denotes |
DNase I footprinting analysis revealed two protected regions, one binding the transcription factors SP1 and SP3 and the other hepatocyte nuclear factor 3beta (HNF-3beta) and HNF-1alpha. |
| T7 |
981-1129 |
Sentence |
denotes |
Cotransfection experiments suggest that HNF-3beta, HNF-1alpha, and SP1 are positive regulators of the herein-described human PDX-1 enhancer element. |
| T8 |
1130-1330 |
Sentence |
denotes |
Furthermore, mutations within each motif abolished the binding of the corresponding factor(s) and dramatically impaired the enhancer activity, therefore suggesting cooperativity between these factors. |
