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PubMed:11087757 JSONTXT 33 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-90 Sentence denotes Multiple isozymes of heparan sulfate/heparin GlcNAc N-deacetylase/GlcN N-sulfotransferase.
T2 91-142 Sentence denotes Structure and activity of the fourth member, NDST4.
T3 143-257 Sentence denotes We report the cloning and partial characterization of the fourth member of the vertebrate heparan sulfate/heparin:
T4 258-336 Sentence denotes GlcNAc N-deacetylase/GlcN N-sulfotransferase family, which we designate NDST4.
T5 337-466 Sentence denotes Full-length cDNA clones containing the entire coding region of 872 amino acids were obtained from human and mouse cDNA libraries.
T6 467-581 Sentence denotes The deduced amino acid sequence of NDST4 showed high sequence identity to NDST1, NDST2, and NDST3 in both species.
T7 582-662 Sentence denotes NDST4 maps to human chromosome 4q25-26, very close to NDST3, located at 4q26-27.
T8 663-853 Sentence denotes These observations, taken together with phylogenetic data, suggest that the four NDSTs evolved from a common ancestral gene, which diverged to give rise to two subtypes, NDST3/4 and NDST1/2.
T9 854-1075 Sentence denotes Reverse transcription-polymerase chain reaction analysis of various mouse tissues revealed a restricted pattern of NDST4 mRNA expression when compared with NDST1 and NDST2, which are abundantly and ubiquitously expressed.
T10 1076-1322 Sentence denotes Comparison of the enzymatic properties of the four murine NDSTs revealed striking differences in N-deacetylation and N-sulfation activities; NDST4 had weak deacetylase activity but high sulfotransferase, whereas NDST3 had the opposite properties.
T11 1323-1577 Sentence denotes Molecular modeling of the sulfotransferase domains of the murine and human NDSTs showed varying surface charge distributions within the substrate binding cleft, suggesting that the differences in activity may reflect preferences for different substrates.
T12 1578-1836 Sentence denotes An iterative model of heparan sulfate biosynthesis is suggested in which some NDST isozymes initiate the N-deacetylation and N-sulfation of the chains, whereas others bind to previously modified segments to fill in or extend the section of modified residues.