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PubMed:11087743 JSONTXT 34 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-75 Sentence denotes Characterization of the stromal cell-derived factor-1alpha-heparin complex.
T2 76-181 Sentence denotes The binding of chemokines to glycosaminoglycans is thought to play a crucial role in chemokine functions.
T3 182-480 Sentence denotes It has recently been shown that stromal cell-derived factor-1alpha (SDF-1alpha), a CXC chemokine with potent anti-human immunodeficiency virus activity, binds to heparan sulfate through a typical consensus sequence for heparin recognition (BBXB, where B is a basic residue KHLK, amino acids 24-27).
T4 481-769 Sentence denotes Calculation of the accessible surface, together with the electrostatic potential of the SDF-1alpha dimer, revealed that other amino acids (Arg-41 and Lys-43) are found in the same surface area and contribute to the creation of a positively charged crevice, located at the dimer interface.
T5 770-905 Sentence denotes GRID calculations confirmed that this binding site will be the most energetically favored area for the interaction with sulfate groups.
T6 906-1063 Sentence denotes Site-directed mutagenesis and surface plasmon resonance-based binding assays were used to investigate the structural basis for SDF-1alpha binding to heparin.
T7 1064-1206 Sentence denotes Among the residues clustered in this basic surface area, Lys-24 and Lys-27 have dominant roles and are essential for interaction with heparin.
T8 1207-1328 Sentence denotes Amino acids Arg-41 and Lys-43 participate in the binding but are not strictly required for the interaction to take place.
T9 1329-1547 Sentence denotes Direct binding assays and competition analysis with monoclonal antibodies also permitted us to show that the N-terminal residue (Lys-1), an amino acid critical for receptor activation, is involved in complex formation.
T10 1548-1846 Sentence denotes Binding studies with selectively desulfated heparin, heparin oligosaccharides, and heparitinase-resistant heparan sulfate fragments showed that a minimum size of 12-14 monosaccharide units is required for efficient binding and that 2-O- and N-sulfate groups have a dominant role in the interaction.
T11 1847-1971 Sentence denotes Finally, the heparin-binding site was identified on the crystal structure of SDF-1alpha, and a docking study was undertaken.
T12 1972-2091 Sentence denotes During the energy minimization process, heparin lost its perfect ribbon shape and fitted the protein surface perfectly.
T13 2092-2249 Sentence denotes In the model, Lys-1, Lys-24, Lys-27, and Arg-41 were found to have the major role in binding a polysaccharide fragment consisting of 13 monosaccharide units.