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Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-94 Sentence denotes Human glucocerebrosidase: heterologous expression of active site mutants in murine null cells.
T1 0-94 Sentence denotes Human glucocerebrosidase: heterologous expression of active site mutants in murine null cells.
T1 0-94 Sentence denotes Human glucocerebrosidase: heterologous expression of active site mutants in murine null cells.
TextSentencer_T2 95-286 Sentence denotes Using bioinformatics methods, we have previously identified Glu235 and Glu340 as the putative acid/base catalyst and nucleophile, respectively, in the active site of human glucocerebrosidase.
T2 95-286 Sentence denotes Using bioinformatics methods, we have previously identified Glu235 and Glu340 as the putative acid/base catalyst and nucleophile, respectively, in the active site of human glucocerebrosidase.
T2 95-286 Sentence denotes Using bioinformatics methods, we have previously identified Glu235 and Glu340 as the putative acid/base catalyst and nucleophile, respectively, in the active site of human glucocerebrosidase.
TextSentencer_T3 287-401 Sentence denotes Thus, we undertook site-directed mutagenesis studies to obtain experimental evidence supporting these predictions.
T3 287-401 Sentence denotes Thus, we undertook site-directed mutagenesis studies to obtain experimental evidence supporting these predictions.
T3 287-401 Sentence denotes Thus, we undertook site-directed mutagenesis studies to obtain experimental evidence supporting these predictions.
TextSentencer_T4 402-539 Sentence denotes Recombinant retroviruses were used to express wild-type and E235A and E340A mutant proteins in glucocerebrosidase-deficient murine cells.
T4 402-539 Sentence denotes Recombinant retroviruses were used to express wild-type and E235A and E340A mutant proteins in glucocerebrosidase-deficient murine cells.
T4 402-539 Sentence denotes Recombinant retroviruses were used to express wild-type and E235A and E340A mutant proteins in glucocerebrosidase-deficient murine cells.
TextSentencer_T5 540-625 Sentence denotes In contrast to wild-type enzyme, the mutants were found to be catalytically inactive.
T5 540-625 Sentence denotes In contrast to wild-type enzyme, the mutants were found to be catalytically inactive.
T5 540-625 Sentence denotes In contrast to wild-type enzyme, the mutants were found to be catalytically inactive.
TextSentencer_T6 626-870 Sentence denotes We also report the results of various studies (Western blotting, glycosylation analysis, subcellular fractionation, and confocal microscopy) indicating that the wild-type and mutant enzymes are identically processed and sorted to the lysosomes.
T6 626-870 Sentence denotes We also report the results of various studies (Western blotting, glycosylation analysis, subcellular fractionation, and confocal microscopy) indicating that the wild-type and mutant enzymes are identically processed and sorted to the lysosomes.
T6 626-870 Sentence denotes We also report the results of various studies (Western blotting, glycosylation analysis, subcellular fractionation, and confocal microscopy) indicating that the wild-type and mutant enzymes are identically processed and sorted to the lysosomes.
TextSentencer_T7 871-971 Sentence denotes Thus, enzymatic inactivity of the mutant proteins is not the result of incorrect folding/processing.
T7 871-971 Sentence denotes Thus, enzymatic inactivity of the mutant proteins is not the result of incorrect folding/processing.
T7 871-971 Sentence denotes Thus, enzymatic inactivity of the mutant proteins is not the result of incorrect folding/processing.
TextSentencer_T8 972-1121 Sentence denotes These findings indicate that Glu235 plays a key role in the catalytic machinery of human glucocerebrosidase and may indeed be the acid/base catalyst.
T8 972-1121 Sentence denotes These findings indicate that Glu235 plays a key role in the catalytic machinery of human glucocerebrosidase and may indeed be the acid/base catalyst.
T8 972-1121 Sentence denotes These findings indicate that Glu235 plays a key role in the catalytic machinery of human glucocerebrosidase and may indeed be the acid/base catalyst.
TextSentencer_T9 1122-1331 Sentence denotes As concerns Glu340, the results both support our computer-based predictions and confirm, at the biological level, previous identification of Glu340 as the nucleophile by use of active site labeling techniques.
T9 1122-1331 Sentence denotes As concerns Glu340, the results both support our computer-based predictions and confirm, at the biological level, previous identification of Glu340 as the nucleophile by use of active site labeling techniques.
T9 1122-1331 Sentence denotes As concerns Glu340, the results both support our computer-based predictions and confirm, at the biological level, previous identification of Glu340 as the nucleophile by use of active site labeling techniques.
TextSentencer_T10 1332-1500 Sentence denotes Finally, our findings may help to better understand the molecular basis of Gaucher disease, the human lysosomal disease resulting from deficiency in glucocerebrosidase.
T10 1332-1500 Sentence denotes Finally, our findings may help to better understand the molecular basis of Gaucher disease, the human lysosomal disease resulting from deficiency in glucocerebrosidase.
T10 1332-1500 Sentence denotes Finally, our findings may help to better understand the molecular basis of Gaucher disease, the human lysosomal disease resulting from deficiency in glucocerebrosidase.