| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-149 |
Sentence |
denotes |
Erk1/2-dependent phosphorylation of Galpha-interacting protein stimulates its GTPase accelerating activity and autophagy in human colon cancer cells. |
| T2 |
150-323 |
Sentence |
denotes |
Galpha-interacting protein (GAIP) is a regulator of G protein signaling (RGS) that accelerates the rate of GTP hydrolysis by the alpha-subunit of the trimeric G(i3) protein. |
| T3 |
324-450 |
Sentence |
denotes |
Both proteins are part of a signaling pathway that controls lysosomal-autophagic catabolism in human colon cancer HT-29 cells. |
| T4 |
451-656 |
Sentence |
denotes |
Here we show that GAIP is phosphorylated by an extracellular signal-regulated (Erk1/2) MAP kinase-dependent pathway sensitive to amino acids, MEK1/2 (PD098059), and protein kinase C (GF109203X) inhibitors. |
| T5 |
657-992 |
Sentence |
denotes |
An in vitro phosphorylation assay demonstrates that Erk2-dependent phosphorylation of GAIP stimulates its GTPase-activating protein activity toward the Galpha(i3) protein (k = 0.187 +/- 0.001 s(-)(1), EC(50) = 1.12 +/- 0.10 microm) when compared with unphosphorylated recombinant GAIP (k = 0.145 +/- 0.003 s(-)(1), EC(50) = 3.16 +/- 0. |
| T6 |
993-1100 |
Sentence |
denotes |
12 microm) or to GAIP phosphorylated by other Ser/Thr protein kinases (protein kinase C, casein kinase II). |
| T7 |
1101-1289 |
Sentence |
denotes |
This stimulation and the phosphorylation of GAIP by Erk2 were abrogated when serine at position 151 in the RGS domain was substituted by an alanine residue using site-directed mutagenesis. |
| T8 |
1290-1444 |
Sentence |
denotes |
Furthermore, the lysosomal-autophagic pathway was not stimulated in S151A-GAIP mutant-expressing cells when compared with wild-type GAIP-expressing cells. |
| T9 |
1445-1561 |
Sentence |
denotes |
These results demonstrate that the GTPase-activating protein activity of GAIP is stimulated by Erk2 phosphorylation. |
| T10 |
1562-1697 |
Sentence |
denotes |
They also suggested that Erk1/2 and GAIP are engaged in the signaling control of a major catabolic pathway in intestinal derived cells. |
