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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-95 Sentence denotes Preparative synthesis of GDP-beta-L-fucose by recombinant enzymes from enterobacterial sources.
T1 0-95 Sentence denotes Preparative synthesis of GDP-beta-L-fucose by recombinant enzymes from enterobacterial sources.
T1 0-95 Sentence denotes Preparative synthesis of GDP-beta-L-fucose by recombinant enzymes from enterobacterial sources.
TextSentencer_T2 96-322 Sentence denotes The 6-deoxyhexose L-fucose is an important and characteristic element in glycoconjugates of bacteria (e.g., lipopolysaccharides), plants (e.g., xyloglucans) and animals (e.g., glycolipids, glycoproteins, and oligosaccharides).
T2 96-322 Sentence denotes The 6-deoxyhexose L-fucose is an important and characteristic element in glycoconjugates of bacteria (e.g., lipopolysaccharides), plants (e.g., xyloglucans) and animals (e.g., glycolipids, glycoproteins, and oligosaccharides).
T2 96-322 Sentence denotes The 6-deoxyhexose L-fucose is an important and characteristic element in glycoconjugates of bacteria (e.g., lipopolysaccharides), plants (e.g., xyloglucans) and animals (e.g., glycolipids, glycoproteins, and oligosaccharides).
TextSentencer_T3 323-643 Sentence denotes The biosynthetic pathway of GDP-L-fucose starts with a dehydration of GDP-D-mannose catalyzed by GDP-D-mannose 4,6-dehydratase (Gmd) creating GDP-4-keto-6-deoxymannose which is subsequently converted by the GDP-4-keto-6-deoxy-D-mannose 3,5-epimerase-4-reductase (WcaG; GDP-beta-L-fucose synthetase) to GDP-beta-L-fucose.
T3 323-643 Sentence denotes The biosynthetic pathway of GDP-L-fucose starts with a dehydration of GDP-D-mannose catalyzed by GDP-D-mannose 4,6-dehydratase (Gmd) creating GDP-4-keto-6-deoxymannose which is subsequently converted by the GDP-4-keto-6-deoxy-D-mannose 3,5-epimerase-4-reductase (WcaG; GDP-beta-L-fucose synthetase) to GDP-beta-L-fucose.
T3 323-643 Sentence denotes The biosynthetic pathway of GDP-L-fucose starts with a dehydration of GDP-D-mannose catalyzed by GDP-D-mannose 4,6-dehydratase (Gmd) creating GDP-4-keto-6-deoxymannose which is subsequently converted by the GDP-4-keto-6-deoxy-D-mannose 3,5-epimerase-4-reductase (WcaG; GDP-beta-L-fucose synthetase) to GDP-beta-L-fucose.
TextSentencer_T4 644-901 Sentence denotes Both biosynthetic genes gmd and wcaG were cloned from Escherichia coli K12 and the enzymes overexpressed under control of the T7 promoter in the expression vectors pET11a and pET16b, yielding both native and N-terminal His-tag fusion proteins, respectively.
T4 644-901 Sentence denotes Both biosynthetic genes gmd and wcaG were cloned from Escherichia coli K12 and the enzymes overexpressed under control of the T7 promoter in the expression vectors pET11a and pET16b, yielding both native and N-terminal His-tag fusion proteins, respectively.
T4 644-901 Sentence denotes Both biosynthetic genes gmd and wcaG were cloned from Escherichia coli K12 and the enzymes overexpressed under control of the T7 promoter in the expression vectors pET11a and pET16b, yielding both native and N-terminal His-tag fusion proteins, respectively.
TextSentencer_T5 902-951 Sentence denotes The activities of the Gmd and WcaG were analyzed.
T5 902-951 Sentence denotes The activities of the Gmd and WcaG were analyzed.
T5 902-951 Sentence denotes The activities of the Gmd and WcaG were analyzed.
TextSentencer_T6 952-1066 Sentence denotes The enzymatic conversion from GDP-D-mannose to GDP-beta-L-fucose was optimized and the final product was purified.
T6 952-1066 Sentence denotes The enzymatic conversion from GDP-D-mannose to GDP-beta-L-fucose was optimized and the final product was purified.
T6 952-1066 Sentence denotes The enzymatic conversion from GDP-D-mannose to GDP-beta-L-fucose was optimized and the final product was purified.
TextSentencer_T7 1067-1167 Sentence denotes The formation of GDP-beta-L-fucose by the recombinant enzymes was verified by HPLC and NMR analyses.
T7 1067-1167 Sentence denotes The formation of GDP-beta-L-fucose by the recombinant enzymes was verified by HPLC and NMR analyses.
T7 1067-1167 Sentence denotes The formation of GDP-beta-L-fucose by the recombinant enzymes was verified by HPLC and NMR analyses.
TextSentencer_T8 1168-1259 Sentence denotes The His-tag fusion variants of the Gmd and WcaG proteins were purified to near homogeneity.
T8 1168-1259 Sentence denotes The His-tag fusion variants of the Gmd and WcaG proteins were purified to near homogeneity.
T8 1168-1259 Sentence denotes The His-tag fusion variants of the Gmd and WcaG proteins were purified to near homogeneity.
TextSentencer_T9 1260-1423 Sentence denotes The His-tag Gmd recombinant enzyme was inactive, whereas His-tag WcaG showed very similar enzymatic properties relative to the native GDP-beta-L-fucose synthetase.
T9 1260-1423 Sentence denotes The His-tag Gmd recombinant enzyme was inactive, whereas His-tag WcaG showed very similar enzymatic properties relative to the native GDP-beta-L-fucose synthetase.
T9 1260-1423 Sentence denotes The His-tag Gmd recombinant enzyme was inactive, whereas His-tag WcaG showed very similar enzymatic properties relative to the native GDP-beta-L-fucose synthetase.
TextSentencer_T10 1424-1772 Sentence denotes With the purified His-tag WcaG Km and Vmax values, respectively, of 40 microM and 23 nkat/mg protein for the substrate GDP-4-keto-6-deoxy-D-mannose and of 21 microM and 10 nkat/mg protein for the cosubstrate NADPH were obtained; a pH optimum of 7.5 was determined and the enzyme was stimulated to equal extend by the divalent cations Mg2+ and Ca2+.
T10 1424-1772 Sentence denotes With the purified His-tag WcaG Km and Vmax values, respectively, of 40 microM and 23 nkat/mg protein for the substrate GDP-4-keto-6-deoxy-D-mannose and of 21 microM and 10 nkat/mg protein for the cosubstrate NADPH were obtained; a pH optimum of 7.5 was determined and the enzyme was stimulated to equal extend by the divalent cations Mg2+ and Ca2+.
T10 1424-1772 Sentence denotes With the purified His-tag WcaG Km and Vmax values, respectively, of 40 microM and 23 nkat/mg protein for the substrate GDP-4-keto-6-deoxy-D-mannose and of 21 microM and 10 nkat/mg protein for the cosubstrate NADPH were obtained; a pH optimum of 7.5 was determined and the enzyme was stimulated to equal extend by the divalent cations Mg2+ and Ca2+.
TextSentencer_T11 1773-1845 Sentence denotes The Gmd enzyme showed a strong feedback inhibition by GDP-beta-L-fucose.
T11 1773-1845 Sentence denotes The Gmd enzyme showed a strong feedback inhibition by GDP-beta-L-fucose.
T11 1773-1845 Sentence denotes The Gmd enzyme showed a strong feedback inhibition by GDP-beta-L-fucose.