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PubMed:10967092 JSONTXT 22 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-122 Sentence denotes Biochemical characterization of an ATPase activity associated with the large packaging subunit gp17 from bacteriophage T4.
T2 123-373 Sentence denotes Double-stranded DNA-packaging in icosahedral bacteriophages is believed to be driven by a packaging "machine" constituted by the portal protein and the two packaging/terminase proteins assembled at the unique portal vertex of the empty prohead shell.
T3 374-545 Sentence denotes Although ATP hydrolysis is evidently the principal driving force, which component of the packaging machinery functions as the translocating ATPase has not been elucidated.
T4 546-648 Sentence denotes Evidence suggests that the large packaging subunit is a strong candidate for the translocating ATPase.
T5 649-835 Sentence denotes We have constructed new phage T4 terminase recombinants under the control of phage T7 promoter and overexpressed the packaging/terminase proteins gp16 and gp17 in various configurations.
T6 836-1009 Sentence denotes The hexahistidine-tagged-packaging proteins were purified to near homogeneity by Ni(2+)-agarose chromatography and were shown to be highly active for packaging DNA in vitro.
T7 1010-1103 Sentence denotes The large packaging subunit gp17 but not the small subunit gp16 exhibited an ATPase activity.
T8 1104-1202 Sentence denotes Although gp16 lacked ATPase activity, it enhanced the gp17-associated ATPase activity by >50-fold.
T9 1203-1299 Sentence denotes The gp16 enhancement was specific and was due to an increased catalytic rate for ATP hydrolysis.
T10 1300-1438 Sentence denotes A phosphorylated gp17 was demonstrated under conditions of low catalytic rates but not under high catalytic rates in the presence of gp16.
T11 1439-1612 Sentence denotes The data are consistent with the hypothesis that a weak ATPase is transformed into a translocating ATPase of high catalytic capacity after assembly of the packaging machine.