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PubMed:1095566 JSONTXT 49 Projects

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Id Subject Object Predicate Lexical cue
T1 0-99 Sentence denotes Role of undecaprenyl phosphate in synthesis of polymers containing sialic acid in Escherichia coli.
T1 0-99 Sentence denotes Role of undecaprenyl phosphate in synthesis of polymers containing sialic acid in Escherichia coli.
T2 100-348 Sentence denotes Membrane-associated sialytransferase complexes from Escherichia coli K-235 catalyze the incorporation of N-acetylneuraminic acid (NeuNAc) from cytidine 5-monophospho-N-acetylneuraminic acid (CMP-NeuNAc) into polymeric products and a lipid fraction.
T2 100-348 Sentence denotes Membrane-associated sialytransferase complexes from Escherichia coli K-235 catalyze the incorporation of N-acetylneuraminic acid (NeuNAc) from cytidine 5-monophospho-N-acetylneuraminic acid (CMP-NeuNAc) into polymeric products and a lipid fraction.
T3 349-579 Sentence denotes Reconstitution of enzyme activity in lipid-depleted membrane complexes had an absolute dependence on a purified phospholipid which was characterized by high resolution mass spectrometry following dephosphorylation as undecaprenol.
T3 349-579 Sentence denotes Reconstitution of enzyme activity in lipid-depleted membrane complexes had an absolute dependence on a purified phospholipid which was characterized by high resolution mass spectrometry following dephosphorylation as undecaprenol.
T4 580-713 Sentence denotes An identical mass spectrum was obtained on the phosphorylated lipid confirming that the active derivative was undecaprenyl phosphate.
T4 580-713 Sentence denotes An identical mass spectrum was obtained on the phosphorylated lipid confirming that the active derivative was undecaprenyl phosphate.
T5 714-835 Sentence denotes This C55-isoprenoid alcohol accounted for 95% of the polyisoprenol and contained 11 isoprene units, each one unsaturated.
T5 714-835 Sentence denotes This C55-isoprenoid alcohol accounted for 95% of the polyisoprenol and contained 11 isoprene units, each one unsaturated.
T6 836-901 Sentence denotes The remaining 5% was composed of the C60 homologue, dodecaprenol.
T6 836-901 Sentence denotes The remaining 5% was composed of the C60 homologue, dodecaprenol.
T7 902-1069 Sentence denotes Ficarprenyl phosphate, an isomer of undecaprenyl phosphate, also restored enzymatic activity although on a molar basis, it was less active than undecaprenyl phosphate.
T7 902-1069 Sentence denotes Ficarprenyl phosphate, an isomer of undecaprenyl phosphate, also restored enzymatic activity although on a molar basis, it was less active than undecaprenyl phosphate.
T8 1070-1367 Sentence denotes These results provide direct evidence that sialyl polymer synthesis has an obligatory requirement for undecaprenyl phosphate, a membrane-bound lipid coenzyme which functions as an intermediate carrier of glycosyl residues in the biosynthesis of a variety of microbial cell surface polysaccharides.
T8 1070-1367 Sentence denotes These results provide direct evidence that sialyl polymer synthesis has an obligatory requirement for undecaprenyl phosphate, a membrane-bound lipid coenzyme which functions as an intermediate carrier of glycosyl residues in the biosynthesis of a variety of microbial cell surface polysaccharides.
T9 1368-1945 Sentence denotes These data extend further the general class of polysaccharides whose synthesis involves undecaprenyl phosphate and are in accord with the hypothesis that this lipid acts as an intermediate carrier of sialyl residues in sialyl polymer synthesis according to the following reaction: CMP-NeuNAc plus P-undecaprenol in equilibrium NeuNAc-P-undecaprenol plus CMP Although the sialylated lipid remains to be characterized, evidence in support of this conclusion was obtained by kinetic analysis of N-acetylneuraminic acid transfer into the lipid soluble fraction and sialyl polymers.
T9 1368-1945 Sentence denotes These data extend further the general class of polysaccharides whose synthesis involves undecaprenyl phosphate and are in accord with the hypothesis that this lipid acts as an intermediate carrier of sialyl residues in sialyl polymer synthesis according to the following reaction: CMP-NeuNAc plus P-undecaprenol in equilibrium NeuNAc-P-undecaprenol plus CMP Although the sialylated lipid remains to be characterized, evidence in support of this conclusion was obtained by kinetic analysis of N-acetylneuraminic acid transfer into the lipid soluble fraction and sialyl polymers.
T10 1946-2221 Sentence denotes These studies showed a rapid incorporation of N-acetylneuraminic acid into the lipid-soluble fraction prior to maximal sialyl polymer formation, an observation consistent with a possible precursortion, an observation consistent with a possible precursor-product relationship.
T10 1946-2221 Sentence denotes These studies showed a rapid incorporation of N-acetylneuraminic acid into the lipid-soluble fraction prior to maximal sialyl polymer formation, an observation consistent with a possible precursortion, an observation consistent with a possible precursor-product relationship.
T11 2222-2483 Sentence denotes Confirmation that the radioactivity in the lipid-soluble fraction was lipid-linked N-acetylneuraminic acid was provided by the demonstration of the formation of a transitory sialyl-lipid with chromatographic properties expected of sialyl-undecaprenyl phosphate.
T11 2222-2483 Sentence denotes Confirmation that the radioactivity in the lipid-soluble fraction was lipid-linked N-acetylneuraminic acid was provided by the demonstration of the formation of a transitory sialyl-lipid with chromatographic properties expected of sialyl-undecaprenyl phosphate.
T12 2484-2610 Sentence denotes Three additional lines of investigation implicated a functional role for undecaprenyl phosphate in sialyl polymer synthesis...
T12 2484-2610 Sentence denotes Three additional lines of investigation implicated a functional role for undecaprenyl phosphate in sialyl polymer synthesis...