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PubMed:10938272 JSONTXT 24 Projects

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Id Subject Object Predicate Lexical cue
T1 0-153 Sentence denotes Rapid induction of histone hyperacetylation and cellular differentiation in human breast tumor cell lines following degradation of histone deacetylase-1.
T2 154-264 Sentence denotes Quinidine inhibits proliferation and promotes cellular differentiation in human breast tumor epithelial cells.
T3 265-419 Sentence denotes Previously we showed quinidine arrested MCF-7 cells in G(1) phase of the cell cycle and led to a G(1) to G(0) transition followed by apoptotic cell death.
T4 420-597 Sentence denotes The present experiments demonstrated that MCF-7, MCF-7ras, T47D, MDA-MB-231, and MDA-MB-435 cells transiently differentiate before undergoing apoptosis in response to quinidine.
T5 598-688 Sentence denotes The cells accumulated lipid droplets, and the cytokeratin 18 cytoskeleton was reorganized.
T6 689-812 Sentence denotes Hyperacetylated histone H4 appeared within 2 h of the addition of quinidine to the medium, and levels were maximal by 24 h.
T7 813-1111 Sentence denotes Quinidine-treated MCF-7 cells showed elevated p21(WAF1), hypophosphorylation and suppression of retinoblastoma protein, and down-regulation of cyclin D1, similar to the cell cycle response observed with cells induced to differentiate by histone deacetylase inhibitors, trichostatin A, and trapoxin.
T8 1112-1217 Sentence denotes Quinidine did not show evidence for direct inhibition of histone deacetylase enzymatic activity in vitro.
T9 1218-1312 Sentence denotes HDAC1 was undetectable in MCF-7 cells 30 min after addition of quinidine to the growth medium.
T10 1313-1418 Sentence denotes The proteasome inhibitors MG-132 and lactacystin completely protected HDAC1 from the action of quinidine.
T11 1419-1559 Sentence denotes We conclude that quinidine is a breast tumor cell differentiating agent that causes the loss of HDAC1 via a proteasomal sensitive mechanism.