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PubMed:10814705 JSONTXT 68 Projects

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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-67 Sentence denotes In vitro characterization of anti-glucosylceramide rabbit antisera.
T1 0-67 Sentence denotes In vitro characterization of anti-glucosylceramide rabbit antisera.
T1 0-67 Sentence denotes In vitro characterization of anti-glucosylceramide rabbit antisera.
TextSentencer_T2 68-145 Sentence denotes Glucosylceramides (GlcCer) are biosynthetic precursors of glycosphingolipids.
T2 68-145 Sentence denotes Glucosylceramides (GlcCer) are biosynthetic precursors of glycosphingolipids.
T2 68-145 Sentence denotes Glucosylceramides (GlcCer) are biosynthetic precursors of glycosphingolipids.
TextSentencer_T3 146-245 Sentence denotes They are widely distributed in biological systems where they exhibit numerous biological functions.
T3 146-245 Sentence denotes They are widely distributed in biological systems where they exhibit numerous biological functions.
T3 146-245 Sentence denotes They are widely distributed in biological systems where they exhibit numerous biological functions.
TextSentencer_T4 246-422 Sentence denotes Studies on the localization of glucosylceramides in different tissues have used biochemical methods only since specific antibodies against GlcCer were not previously available.
T4 246-422 Sentence denotes Studies on the localization of glucosylceramides in different tissues have used biochemical methods only since specific antibodies against GlcCer were not previously available.
T4 246-422 Sentence denotes Studies on the localization of glucosylceramides in different tissues have used biochemical methods only since specific antibodies against GlcCer were not previously available.
TextSentencer_T5 423-720 Sentence denotes We have characterized two commercially available rabbit antisera which were prepared against GlcCer of plant origin (1-O-(beta-D-glucopyranosyl)-N-acyl-4-hydroxysphinganine; GlcCer-3) or human origin (1-O-(beta-D-glucopyranosyl)-N-acyl-sphingosine; GlcCer-2) and claimed to be specific for GlcCer.
T5 423-720 Sentence denotes We have characterized two commercially available rabbit antisera which were prepared against GlcCer of plant origin (1-O-(beta-D-glucopyranosyl)-N-acyl-4-hydroxysphinganine; GlcCer-3) or human origin (1-O-(beta-D-glucopyranosyl)-N-acyl-sphingosine; GlcCer-2) and claimed to be specific for GlcCer.
T5 423-720 Sentence denotes We have characterized two commercially available rabbit antisera which were prepared against GlcCer of plant origin (1-O-(beta-D-glucopyranosyl)-N-acyl-4-hydroxysphinganine; GlcCer-3) or human origin (1-O-(beta-D-glucopyranosyl)-N-acyl-sphingosine; GlcCer-2) and claimed to be specific for GlcCer.
TextSentencer_T6 721-878 Sentence denotes The antisera were also able to detect specifically GlcCer species in crude lipid extracts from human epidermis after separation by thin-layer chromatography.
T6 721-878 Sentence denotes The antisera were also able to detect specifically GlcCer species in crude lipid extracts from human epidermis after separation by thin-layer chromatography.
T6 721-878 Sentence denotes The antisera were also able to detect specifically GlcCer species in crude lipid extracts from human epidermis after separation by thin-layer chromatography.
TextSentencer_T7 879-1065 Sentence denotes The reagents are sensitive since both antisera reacted at dilutions higher than 1:500 with their homologous antigen in the nanogram range in thin layer immunostaining or dot-blot assays.
T7 879-1065 Sentence denotes The reagents are sensitive since both antisera reacted at dilutions higher than 1:500 with their homologous antigen in the nanogram range in thin layer immunostaining or dot-blot assays.
T7 879-1065 Sentence denotes The reagents are sensitive since both antisera reacted at dilutions higher than 1:500 with their homologous antigen in the nanogram range in thin layer immunostaining or dot-blot assays.
TextSentencer_T8 1066-1216 Sentence denotes The antisera are specific for GlcCer although they did not differentiate between GlcCer-2 and GlcCer-3 containing sphingosine or 4-hydroxysphinganine.
T8 1066-1216 Sentence denotes The antisera are specific for GlcCer although they did not differentiate between GlcCer-2 and GlcCer-3 containing sphingosine or 4-hydroxysphinganine.
T8 1066-1216 Sentence denotes The antisera are specific for GlcCer although they did not differentiate between GlcCer-2 and GlcCer-3 containing sphingosine or 4-hydroxysphinganine.
TextSentencer_T9 1217-1413 Sentence denotes The antisera also reacted with N-stearoyl-DL-dihydroglucocere-broside indicating that the naturally occurring structural variations in the amino alcohol moiety are not determining the specificity.
T9 1217-1413 Sentence denotes The antisera also reacted with N-stearoyl-DL-dihydroglucocere-broside indicating that the naturally occurring structural variations in the amino alcohol moiety are not determining the specificity.
T9 1217-1413 Sentence denotes The antisera also reacted with N-stearoyl-DL-dihydroglucocere-broside indicating that the naturally occurring structural variations in the amino alcohol moiety are not determining the specificity.
TextSentencer_T10 1414-1623 Sentence denotes No crossreactivity was observed with other mono- or diglycosylceramides (galactosylceramides, lactosyl-ceramide), free ceramides or structurally unrelated lipids (cholesterol, sphingomyelin, or phospholipids).
T10 1414-1623 Sentence denotes No crossreactivity was observed with other mono- or diglycosylceramides (galactosylceramides, lactosyl-ceramide), free ceramides or structurally unrelated lipids (cholesterol, sphingomyelin, or phospholipids).
T10 1414-1623 Sentence denotes No crossreactivity was observed with other mono- or diglycosylceramides (galactosylceramides, lactosyl-ceramide), free ceramides or structurally unrelated lipids (cholesterol, sphingomyelin, or phospholipids).
TextSentencer_T11 1624-1705 Sentence denotes Therefore, the glycosylmoiety seems to represent the major antigenic determinant.
T11 1624-1705 Sentence denotes Therefore, the glycosylmoiety seems to represent the major antigenic determinant.
T11 1624-1705 Sentence denotes Therefore, the glycosylmoiety seems to represent the major antigenic determinant.
TextSentencer_T12 1706-1934 Sentence denotes Finally, the antisera also proved to be useful for the immunohistochemical localization of GlcCer in human epidermis by which earlier biochemical data on the distribution of GlcCer in the various epidermal layers were confirmed.
T12 1706-1934 Sentence denotes Finally, the antisera also proved to be useful for the immunohistochemical localization of GlcCer in human epidermis by which earlier biochemical data on the distribution of GlcCer in the various epidermal layers were confirmed.
T12 1706-1934 Sentence denotes Finally, the antisera also proved to be useful for the immunohistochemical localization of GlcCer in human epidermis by which earlier biochemical data on the distribution of GlcCer in the various epidermal layers were confirmed.