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PubMed:10791963 JSONTXT 24 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-61 Sentence denotes Cytoplasmic, nuclear, and golgi localization of RGS proteins.
T2 62-145 Sentence denotes Evidence for N-terminal and RGS domain sequences as intracellular targeting motifs.
T3 146-271 Sentence denotes RGS proteins comprise a family of proteins named for their ability to negatively regulate heterotrimeric G protein signaling.
T4 272-518 Sentence denotes Biochemical studies suggest that members of this protein family act as GTPase-activating proteins for certain Galpha subunits, thereby accelerating the turn-off mechanism of Galpha and terminating signaling by both Galpha and Gbetagamma subunits.
T5 519-763 Sentence denotes In the present study, we used confocal microscopy to examine the intracellular distribution of several RGS proteins in COS-7 cells expressing RGS-green fluorescent protein (GFP) fusion proteins and in cells expressing RGS proteins endogenously.
T6 764-871 Sentence denotes RGS2 and RGS10 accumulated in the nucleus of COS-7 cells transfected with GFP constructs of these proteins.
T7 872-952 Sentence denotes In contrast, RGS4 and RGS16 accumulated in the cytoplasm of COS-7 transfectants.
T8 953-1114 Sentence denotes As observed in COS-7 cells, RGS4 exhibited cytoplasmic localization in mouse neuroblastoma cells, and RGS10 exhibited nuclear localization in human glioma cells.
T9 1115-1361 Sentence denotes Deletion or alanine substitution of an N-terminal leucine repeat motif present in both RGS4 and RGS16, a domain identified as a nuclear export sequence in HIV Rev and other proteins, promoted nuclear localization of these proteins in COS-7 cells.
T10 1362-1565 Sentence denotes In agreement with this observation, treatment of mouse neuroblastoma cells with leptomycin B to inhibit nuclear protein export by exportin1 resulted in accumulation of RGS4 in the nucleus of these cells.
T11 1566-1744 Sentence denotes GFP fusions of RGS domains of RGS proteins localized in the nucleus, suggesting that nuclear localization of RGS proteins results from nuclear targeting via RGS domain sequences.
T12 1745-1873 Sentence denotes RGSZ, which shares with RGS-GAIP a cysteine-rich string in its N-terminal region, localized to the Golgi complex in COS-7 cells.
T13 1874-1987 Sentence denotes Deletion of the N-terminal domain of RGSZ that includes the cysteine motif promoted nuclear localization of RGSZ.
T14 1988-2060 Sentence denotes None of the RGS proteins examined were localized at the plasma membrane.
T15 2061-2229 Sentence denotes These results demonstrate that RGS proteins localize in the nucleus, the cytoplasm, or shuttle between the nucleus and cytoplasm as nucleo-cytoplasmic shuttle proteins.
T16 2230-2429 Sentence denotes RGS proteins localize differentially within cells as a result of structural differences among these proteins that do not appear to be important determinants for their G protein-regulating activities.
T17 2430-2542 Sentence denotes These findings suggest involvement of RGS proteins in more complex cellular functions than currently envisioned.