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PubMed:10781596 JSONTXT 40 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-77 Sentence denotes Molecular cloning and expression of a novel chondroitin 6-O-sulfotransferase.
T2 78-308 Sentence denotes A novel human chondroitin 6-O-sulfotransferase, designated C6ST-2, was identified by BLAST analysis of expressed sequence tag using the sequence of a previously described human chondroitin 6-O-sulfotransferase (C6ST-1) as a probe.
T3 309-448 Sentence denotes The new cDNA sequence revealed an open reading frame coding for a protein of 486 amino acids with a type II transmembrane protein topology.
T4 449-599 Sentence denotes The amino acid sequence displayed 24% identity to the human C6ST-1, and the highest sequence identity was found in the COOH-terminal catalytic domain.
T5 600-759 Sentence denotes The expression of a soluble recombinant form of the protein in COS-1 cells produced an active sulfotransferase with marked specificity for polymer chondroitin.
T6 760-930 Sentence denotes In contrast, keratan sulfate and oligosaccharides containing the Galbeta1-4GlcNAc sequence, which are good acceptor substrates for the C6ST-1, hardly served as acceptors.
T7 931-1108 Sentence denotes The identification of the reaction product indicated that the enzyme is a novel chondroitin 6-O-sulfotransferase (C6ST-2) that mainly transfers sulfate to N-acetylgalactosamine.
T8 1109-1201 Sentence denotes The coding region of C6ST-2 was contained in a single exon and localized to chromosome Xp11.
T9 1202-1306 Sentence denotes Northern blot analysis of human brain poly(A)(+) RNA revealed a single transcript of 2.4 kilobase pairs.
T10 1307-1490 Sentence denotes Reverse transcription-polymerase chain reaction analysis showed that C6ST-2 is developmentally regulated in various tissues with expression persisting through adulthood in the spleen.
T11 1491-1809 Sentence denotes Thus, we demonstrated the redundancy in chondroitin 6-O-sulfotransferases capable of forming chondroitin 6-sulfate, which is important for understanding the mechanisms leading to specific changes in the sulfation profile of chondroitin sulfate chains in various tissues during development and malignant transformation.