| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-149 |
Sentence |
denotes |
Bicarbonate binding activity of the CmpA protein of the cyanobacterium Synechococcus sp. strain PCC 7942 involved in active transport of bicarbonate. |
| T2 |
150-298 |
Sentence |
denotes |
The cmpABCD operon of the cyanobacterium Synechococcus sp. strain PCC 7942 encodes an ATP-binding cassette transporter involved in HCO(3)(-) uptake. |
| T3 |
299-562 |
Sentence |
denotes |
The three genes, cmpBCD, encode membrane components of an ATP-binding cassette transporter, whereas cmpA encodes a 42-kDa cytoplasmic membrane protein, which is 46.5% identical to the membrane-anchored substrate-binding protein of the nitrate/nitrite transporter. |
| T4 |
563-821 |
Sentence |
denotes |
Equilibrium dialysis analysis using H(14)CO(3)(-) showed that a truncated CmpA protein lacking the N-terminal 31 amino acids, expressed in Escherichia coli cells as a histidine-tagged soluble protein, specifically binds inorganic carbon (CO(2) or HCO(3)(-)). |
| T5 |
822-995 |
Sentence |
denotes |
The addition of the recombinant CmpA protein to a buffer caused a decrease in the concentration of dissolved CO(2) because of the binding of inorganic carbon to the protein. |
| T6 |
996-1150 |
Sentence |
denotes |
The decrease in CO(2) concentration was accelerated by the addition of carbonic anhydrase, indicating that HCO(3)(-), but not CO(2), binds to the protein. |
| T7 |
1151-1369 |
Sentence |
denotes |
Mass spectrometric measurements of the amounts of unbound and bound HCO(3)(-) in CmpA solutions containing low concentrations of inorganic carbon revealed that CmpA binds HCO(3)(-) with high affinity (K(d) = 5 microm). |
| T8 |
1370-1596 |
Sentence |
denotes |
A similar dissociation constant was obtained by analysis of the competitive inhibition of the CmpA protein on the carboxylation of phosphoenolpyruvate by phosphoenolpyruvate carboxylase at limiting concentrations of HCO(3)(-). |
| T9 |
1597-1705 |
Sentence |
denotes |
These findings showed that the cmpA gene encodes the substrate-binding protein of the HCO(3)(-) transporter. |
