| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-88 |
Sentence |
denotes |
Direct transactivation of the anti-apoptotic gene apolipoprotein J (clusterin) by B-MYB. |
| T2 |
89-224 |
Sentence |
denotes |
B-MYB is a ubiquitously expressed transcription factor involved in the regulation of cell survival, proliferation, and differentiation. |
| T3 |
225-451 |
Sentence |
denotes |
In an attempt to isolate B-MYB-regulated genes that may explain the role of B-MYB in cellular processes, representational difference analysis was performed in neuroblastoma cell lines with different levels of B-MYB expression. |
| T4 |
452-658 |
Sentence |
denotes |
One of the genes, the mRNA levels of which were enhanced in B-MYB expressing cells, was ApoJ/Clusterin(SGP-2/TRMP-2) (ApoJ/Clusterin), previously implicated in regulation of apoptosis and tumor progression. |
| T5 |
659-930 |
Sentence |
denotes |
Here we show that the human ApoJ/Clusterin gene contains a Myb binding site in its 5' flanking region, which interacts with bacterially synthesized B-MYB protein and mediates B-MYB-dependent transactivation of the ApoJ/Clusterin promoter in transient transfection assays. |
| T6 |
931-1052 |
Sentence |
denotes |
Endogenous ApoJ/Clusterin expression is induced in mammalian cell lines following transient transfection of a B-MYB cDNA. |
| T7 |
1053-1212 |
Sentence |
denotes |
Blockage of secreted clusterin by a monoclonal antibody results in increased apoptosis of neuroblastoma cells exposed to the chemotherapeutic drug doxorubicin. |
| T8 |
1213-1342 |
Sentence |
denotes |
Thus, activation of ApoJ/Clusterin by B-MYB may be an important step in the regulation of apoptosis in normal and diseased cells. |
