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Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-133 Sentence denotes A sequence motif involved in the donor substrate binding by alpha1,6-fucosyltransferase: the role of the conserved arginine residues.
T1 0-133 Sentence denotes A sequence motif involved in the donor substrate binding by alpha1,6-fucosyltransferase: the role of the conserved arginine residues.
TextSentencer_T2 134-258 Sentence denotes Alpha1,6-fucosyltransferase catalyzes the transfer of fucose to the innermost GlcNAc residue of an N-linked oligosaccharide.
T2 134-258 Sentence denotes Alpha1,6-fucosyltransferase catalyzes the transfer of fucose to the innermost GlcNAc residue of an N-linked oligosaccharide.
TextSentencer_T3 259-559 Sentence denotes In order to identify the amino acid residue(s) which are associated with the enzyme activity and to investigate their function, we prepared a series of mutant human alpha1,6-fucosyltransferases in which the conserved residues in the region homologous to alpha1,2-fucosyltransferase had been replaced.
T3 259-559 Sentence denotes In order to identify the amino acid residue(s) which are associated with the enzyme activity and to investigate their function, we prepared a series of mutant human alpha1,6-fucosyltransferases in which the conserved residues in the region homologous to alpha1,2-fucosyltransferase had been replaced.
TextSentencer_T4 560-619 Sentence denotes These proteins were then characterized by kinetic analyses.
T4 560-619 Sentence denotes These proteins were then characterized by kinetic analyses.
TextSentencer_T5 620-728 Sentence denotes The wild-type and mutant alpha1,6-fucosyltransferases were expressed using a baculovirus-insect cell system.
T5 620-728 Sentence denotes The wild-type and mutant alpha1,6-fucosyltransferases were expressed using a baculovirus-insect cell system.
TextSentencer_T6 729-950 Sentence denotes The activity assay showed that replacement of Arg-365 by Ala or Lys led to a complete loss of activity while substitution of Ala or Lys for the neighboring Arg-366 decreased the activity to about 3% that of the wild type.
T6 729-950 Sentence denotes The activity assay showed that replacement of Arg-365 by Ala or Lys led to a complete loss of activity while substitution of Ala or Lys for the neighboring Arg-366 decreased the activity to about 3% that of the wild type.
TextSentencer_T7 951-1160 Sentence denotes Kinetic analyses revealed that the replacements of Arg-366 lead to an increase in the apparent K (m) value for both GDP-fucose and the acceptor oligosaccharide but did not markedly affect the apparent V (max).
T7 951-1160 Sentence denotes Kinetic analyses revealed that the replacements of Arg-366 lead to an increase in the apparent K (m) value for both GDP-fucose and the acceptor oligosaccharide but did not markedly affect the apparent V (max).
TextSentencer_T8 1161-1349 Sentence denotes When these mutants were inhibited by GDP in a competitive manner with respect to the donor substrate, the K (i) values were found to be 50-100 times higher than the value in the wild type.
T8 1161-1349 Sentence denotes When these mutants were inhibited by GDP in a competitive manner with respect to the donor substrate, the K (i) values were found to be 50-100 times higher than the value in the wild type.
TextSentencer_T9 1350-1471 Sentence denotes On the other hand, in the inhibition by GMP, the K (i) values for the mutants were very similar to that of the wild type.
T9 1350-1471 Sentence denotes On the other hand, in the inhibition by GMP, the K (i) values for the mutants were very similar to that of the wild type.
TextSentencer_T10 1472-1701 Sentence denotes These findings suggest that Arg-366 contributes to the binding of GDP-fucose via an interaction with the beta-phosphoryl group of the GDP moiety of the donor, and that Arg-365 may also play an essential role in substrate binding.
T10 1472-1701 Sentence denotes These findings suggest that Arg-366 contributes to the binding of GDP-fucose via an interaction with the beta-phosphoryl group of the GDP moiety of the donor, and that Arg-365 may also play an essential role in substrate binding.
TextSentencer_T11 1702-1849 Sentence denotes The results suggest that the motif common to alpha1,2- and alpha1,6-fucosyltransferases is critical for binding of the donor substrate, GDP-fucose.
T11 1702-1849 Sentence denotes The results suggest that the motif common to alpha1,2- and alpha1,6-fucosyltransferases is critical for binding of the donor substrate, GDP-fucose.