| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-63 |
Sentence |
denotes |
Remodeling of sugar chain structures of human interferon-gamma. |
| T1 |
0-63 |
Sentence |
denotes |
Remodeling of sugar chain structures of human interferon-gamma. |
| T1 |
0-63 |
Sentence |
denotes |
Remodeling of sugar chain structures of human interferon-gamma. |
| TextSentencer_T2 |
64-193 |
Sentence |
denotes |
Natural human interferon (IFN)-gamma has mainly biantennary complex-type sugar chains and scarcely has multiantennary structures. |
| T2 |
64-193 |
Sentence |
denotes |
Natural human interferon (IFN)-gamma has mainly biantennary complex-type sugar chains and scarcely has multiantennary structures. |
| T2 |
64-193 |
Sentence |
denotes |
Natural human interferon (IFN)-gamma has mainly biantennary complex-type sugar chains and scarcely has multiantennary structures. |
| TextSentencer_T3 |
194-285 |
Sentence |
denotes |
We attempted to remodel the sugar chain structures using IFN-gamma as a model glycoprotein. |
| T3 |
194-285 |
Sentence |
denotes |
We attempted to remodel the sugar chain structures using IFN-gamma as a model glycoprotein. |
| T3 |
194-285 |
Sentence |
denotes |
We attempted to remodel the sugar chain structures using IFN-gamma as a model glycoprotein. |
| TextSentencer_T4 |
286-628 |
Sentence |
denotes |
To obtain the branching glycoforms of IFN-gamma, we introduced the genes for GnT-IV (UDP-N-acetylglucosamine:alpha-1,3-D-mannoside beta-1, 4-N-acetylglucosaminyltransferase) and/or GnT-V (UDP-N-acetylglucosamine:alpha-1,6-D-mannoside beta-1, 6-N-acetylglucosaminyltransferase) into Chinese hamster ovary (CHO) cells producing human IFN-gamma. |
| T4 |
286-628 |
Sentence |
denotes |
To obtain the branching glycoforms of IFN-gamma, we introduced the genes for GnT-IV (UDP-N-acetylglucosamine:alpha-1,3-D-mannoside beta-1, 4-N-acetylglucosaminyltransferase) and/or GnT-V (UDP-N-acetylglucosamine:alpha-1,6-D-mannoside beta-1, 6-N-acetylglucosaminyltransferase) into Chinese hamster ovary (CHO) cells producing human IFN-gamma. |
| T4 |
286-628 |
Sentence |
denotes |
To obtain the branching glycoforms of IFN-gamma, we introduced the genes for GnT-IV (UDP-N-acetylglucosamine:alpha-1,3-D-mannoside beta-1, 4-N-acetylglucosaminyltransferase) and/or GnT-V (UDP-N-acetylglucosamine:alpha-1,6-D-mannoside beta-1, 6-N-acetylglucosaminyltransferase) into Chinese hamster ovary (CHO) cells producing human IFN-gamma. |
| TextSentencer_T5 |
629-708 |
Sentence |
denotes |
The parental CHO cells produced IFN-gamma with biantennary sugar chains mainly. |
| T5 |
629-708 |
Sentence |
denotes |
The parental CHO cells produced IFN-gamma with biantennary sugar chains mainly. |
| T5 |
629-708 |
Sentence |
denotes |
The parental CHO cells produced IFN-gamma with biantennary sugar chains mainly. |
| TextSentencer_T6 |
709-856 |
Sentence |
denotes |
When the GnT-IV activity was increased, triantennary sugar chains with a branch produced by GnT-IV increased up to 66.9% of the total sugar chains. |
| T6 |
709-856 |
Sentence |
denotes |
When the GnT-IV activity was increased, triantennary sugar chains with a branch produced by GnT-IV increased up to 66.9% of the total sugar chains. |
| T6 |
709-856 |
Sentence |
denotes |
When the GnT-IV activity was increased, triantennary sugar chains with a branch produced by GnT-IV increased up to 66.9% of the total sugar chains. |
| TextSentencer_T7 |
857-998 |
Sentence |
denotes |
When the GnT-V activity was increased, triantennary sugar chains with a corresponding branch increased up to 55.7% of the total sugar chains. |
| T7 |
857-998 |
Sentence |
denotes |
When the GnT-V activity was increased, triantennary sugar chains with a corresponding branch increased up to 55.7% of the total sugar chains. |
| T7 |
857-998 |
Sentence |
denotes |
When the GnT-V activity was increased, triantennary sugar chains with a corresponding branch increased up to 55.7% of the total sugar chains. |
| TextSentencer_T8 |
999-1135 |
Sentence |
denotes |
When the GnT-IV and -V activities were increased at a time, tetraantennary sugar chains increased up to 56.2% of the total sugar chains. |
| T8 |
999-1135 |
Sentence |
denotes |
When the GnT-IV and -V activities were increased at a time, tetraantennary sugar chains increased up to 56.2% of the total sugar chains. |
| T8 |
999-1135 |
Sentence |
denotes |
When the GnT-IV and -V activities were increased at a time, tetraantennary sugar chains increased up to 56.2% of the total sugar chains. |
| TextSentencer_T9 |
1136-1250 |
Sentence |
denotes |
The proportion of these multiantennary sugar chains corresponded to the intracellular activities of GnT-IV and -V. |
| T9 |
1136-1250 |
Sentence |
denotes |
The proportion of these multiantennary sugar chains corresponded to the intracellular activities of GnT-IV and -V. |
| T9 |
1136-1529 |
Sentence |
denotes |
The proportion of these multiantennary sugar chains corresponded to the intracellular activities of GnT-IV and -V. What is more, lectin blot and flow cytometric analysis indicated that the multi-branch structure of the sugar chains was increased not only on IFN-gamma, one of the secretory glycoproteins, but also on almost CHO cellular proteins by introducing either or both of the GnT genes. |
| TextSentencer_T10 |
1251-1529 |
Sentence |
denotes |
What is more, lectin blot and flow cytometric analysis indicated that the multi-branch structure of the sugar chains was increased not only on IFN-gamma, one of the secretory glycoproteins, but also on almost CHO cellular proteins by introducing either or both of the GnT genes. |
| T10 |
1251-1529 |
Sentence |
denotes |
What is more, lectin blot and flow cytometric analysis indicated that the multi-branch structure of the sugar chains was increased not only on IFN-gamma, one of the secretory glycoproteins, but also on almost CHO cellular proteins by introducing either or both of the GnT genes. |
| TextSentencer_T11 |
1530-1672 |
Sentence |
denotes |
The results suggest that the branching structure of sugar chains of glycoproteins could be controlled by cellular GnT-IV and GnT-V activities. |
| T10 |
1530-1672 |
Sentence |
denotes |
The results suggest that the branching structure of sugar chains of glycoproteins could be controlled by cellular GnT-IV and GnT-V activities. |
| T11 |
1530-1672 |
Sentence |
denotes |
The results suggest that the branching structure of sugar chains of glycoproteins could be controlled by cellular GnT-IV and GnT-V activities. |
| TextSentencer_T12 |
1673-1801 |
Sentence |
denotes |
This technology can produce glycoforms out of natural occurrence, which should enlarge the potency of glycoprotein therapeutics. |
| T11 |
1673-1801 |
Sentence |
denotes |
This technology can produce glycoforms out of natural occurrence, which should enlarge the potency of glycoprotein therapeutics. |
| T12 |
1673-1801 |
Sentence |
denotes |
This technology can produce glycoforms out of natural occurrence, which should enlarge the potency of glycoprotein therapeutics. |