> top > projects > sentences > docs > PubMed:10764822 > annotations
sentences Home  

PubMed:10764822 JSONTXT 54 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-66 Sentence denotes N-Glycan processing by a lepidopteran insect alpha1,2-mannosidase.
T1 0-66 Sentence denotes N-Glycan processing by a lepidopteran insect alpha1,2-mannosidase.
T1 0-66 Sentence denotes N-Glycan processing by a lepidopteran insect alpha1,2-mannosidase.
TextSentencer_T2 67-150 Sentence denotes Protein glycosylation pathways are relatively poorly characterized in insect cells.
T2 67-150 Sentence denotes Protein glycosylation pathways are relatively poorly characterized in insect cells.
T2 67-150 Sentence denotes Protein glycosylation pathways are relatively poorly characterized in insect cells.
TextSentencer_T3 151-368 Sentence denotes As part of an overall effort to address this problem, we previously isolated a cDNA from Sf9 cells that encodes an insect alpha1,2-mannosidase (SfManI) which requires calcium and is inhibited by 1-deoxymannojirimycin.
T3 151-368 Sentence denotes As part of an overall effort to address this problem, we previously isolated a cDNA from Sf9 cells that encodes an insect alpha1,2-mannosidase (SfManI) which requires calcium and is inhibited by 1-deoxymannojirimycin.
T3 151-368 Sentence denotes As part of an overall effort to address this problem, we previously isolated a cDNA from Sf9 cells that encodes an insect alpha1,2-mannosidase (SfManI) which requires calcium and is inhibited by 1-deoxymannojirimycin.
TextSentencer_T4 369-449 Sentence denotes In the present study, we have characterized the substrate specificity of SfManI.
T4 369-449 Sentence denotes In the present study, we have characterized the substrate specificity of SfManI.
T4 369-449 Sentence denotes In the present study, we have characterized the substrate specificity of SfManI.
TextSentencer_T5 450-608 Sentence denotes A recombinant baculovirus was used to express a GST-tagged secreted form of SfManI which was purified from the medium using an immobilized glutathione column.
T5 450-608 Sentence denotes A recombinant baculovirus was used to express a GST-tagged secreted form of SfManI which was purified from the medium using an immobilized glutathione column.
T5 450-608 Sentence denotes A recombinant baculovirus was used to express a GST-tagged secreted form of SfManI which was purified from the medium using an immobilized glutathione column.
TextSentencer_T6 609-729 Sentence denotes The purified SfManI was then incubated with oligosaccharide substrates and the resulting products were analyzed by HPLC.
T6 609-729 Sentence denotes The purified SfManI was then incubated with oligosaccharide substrates and the resulting products were analyzed by HPLC.
T6 609-729 Sentence denotes The purified SfManI was then incubated with oligosaccharide substrates and the resulting products were analyzed by HPLC.
TextSentencer_T7 730-894 Sentence denotes These analyses showed that SfManI rapidly converts Man(9)GlcNAc(2)to Man(6)Glc-NAc(2)isomer C, then more slowly converts Man(6)GlcNAc(2)isomer C to Man(5)GlcNAc(2).
T7 730-894 Sentence denotes These analyses showed that SfManI rapidly converts Man(9)GlcNAc(2)to Man(6)Glc-NAc(2)isomer C, then more slowly converts Man(6)GlcNAc(2)isomer C to Man(5)GlcNAc(2).
T7 730-894 Sentence denotes These analyses showed that SfManI rapidly converts Man(9)GlcNAc(2)to Man(6)Glc-NAc(2)isomer C, then more slowly converts Man(6)GlcNAc(2)isomer C to Man(5)GlcNAc(2).
TextSentencer_T8 895-1052 Sentence denotes The slow step in the processing of Man(9)GlcNAc(2)to Man(5)GlcNAc(2)by SfManI is removal of the alpha1,2-linked mannose on the middle arm of Man(9)GlcNAc(2).
T8 895-1052 Sentence denotes The slow step in the processing of Man(9)GlcNAc(2)to Man(5)GlcNAc(2)by SfManI is removal of the alpha1,2-linked mannose on the middle arm of Man(9)GlcNAc(2).
T8 895-1052 Sentence denotes The slow step in the processing of Man(9)GlcNAc(2)to Man(5)GlcNAc(2)by SfManI is removal of the alpha1,2-linked mannose on the middle arm of Man(9)GlcNAc(2).
TextSentencer_T9 1053-1133 Sentence denotes In this respect, SfManI is similar to mammalian alpha1,2-mannosidases IA and IB.
T9 1053-1133 Sentence denotes In this respect, SfManI is similar to mammalian alpha1,2-mannosidases IA and IB.
T9 1053-1133 Sentence denotes In this respect, SfManI is similar to mammalian alpha1,2-mannosidases IA and IB.
TextSentencer_T10 1134-1375 Sentence denotes However, additional HPLC and(1)H-NMR analyses demonstrated that SfManI converts Man(9)GlcNAc(2)to Man(5)GlcNAc(2)primarily through Man(7)GlcNAc(2)isomer C, the archetypal Man(9)GlcNAc(2)missing the lower arm alpha1,2-linked mannose residues.
T10 1134-1375 Sentence denotes However, additional HPLC and(1)H-NMR analyses demonstrated that SfManI converts Man(9)GlcNAc(2)to Man(5)GlcNAc(2)primarily through Man(7)GlcNAc(2)isomer C, the archetypal Man(9)GlcNAc(2)missing the lower arm alpha1,2-linked mannose residues.
T10 1134-1375 Sentence denotes However, additional HPLC and(1)H-NMR analyses demonstrated that SfManI converts Man(9)GlcNAc(2)to Man(5)GlcNAc(2)primarily through Man(7)GlcNAc(2)isomer C, the archetypal Man(9)GlcNAc(2)missing the lower arm alpha1,2-linked mannose residues.
TextSentencer_T11 1376-1579 Sentence denotes In this respect, SfManI differs from mammalian alpha1,2-mannosidases IA and IB, and is the first alpha1,2-mannosidase directly shown to produce Man(7)GlcNAc(2)isomer C as a major processing intermediate.
T11 1376-1579 Sentence denotes In this respect, SfManI differs from mammalian alpha1,2-mannosidases IA and IB, and is the first alpha1,2-mannosidase directly shown to produce Man(7)GlcNAc(2)isomer C as a major processing intermediate.
T11 1376-1579 Sentence denotes In this respect, SfManI differs from mammalian alpha1,2-mannosidases IA and IB, and is the first alpha1,2-mannosidase directly shown to produce Man(7)GlcNAc(2)isomer C as a major processing intermediate.