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PubMed:10704531 JSONTXT 54 Projects

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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-116 Sentence denotes Cloning of the human cDNA which can complement the defect of the yeast mannosyltransferase I-deficient mutant alg 1.
T1 0-116 Sentence denotes Cloning of the human cDNA which can complement the defect of the yeast mannosyltransferase I-deficient mutant alg 1.
T1 0-116 Sentence denotes Cloning of the human cDNA which can complement the defect of the yeast mannosyltransferase I-deficient mutant alg 1.
TextSentencer_T2 117-260 Sentence denotes The assembly of the lipid-linked oligosaccharide, Glc(3)Man(9)GlcNAc(2)-P-P-Dol, occurs on the rough ER membrane in an ordered stepwise manner.
T2 117-260 Sentence denotes The assembly of the lipid-linked oligosaccharide, Glc(3)Man(9)GlcNAc(2)-P-P-Dol, occurs on the rough ER membrane in an ordered stepwise manner.
T2 117-260 Sentence denotes The assembly of the lipid-linked oligosaccharide, Glc(3)Man(9)GlcNAc(2)-P-P-Dol, occurs on the rough ER membrane in an ordered stepwise manner.
TextSentencer_T3 261-310 Sentence denotes The process is highly conserved among eukaryotes.
T3 261-310 Sentence denotes The process is highly conserved among eukaryotes.
T3 261-310 Sentence denotes The process is highly conserved among eukaryotes.
TextSentencer_T4 311-487 Sentence denotes In order to isolate the human mannosyltransferase I (MT-I) gene involved in the process, we used the Saccharomyces cerevisiae MT-I gene ( ALG1 ), which has already been cloned.
T4 311-487 Sentence denotes In order to isolate the human mannosyltransferase I (MT-I) gene involved in the process, we used the Saccharomyces cerevisiae MT-I gene ( ALG1 ), which has already been cloned.
T4 311-487 Sentence denotes In order to isolate the human mannosyltransferase I (MT-I) gene involved in the process, we used the Saccharomyces cerevisiae MT-I gene ( ALG1 ), which has already been cloned.
TextSentencer_T5 488-616 Sentence denotes On searching the EST database with the amino acid sequence of the ALG1 gene product, we detected seven related human EST clones.
T5 488-616 Sentence denotes On searching the EST database with the amino acid sequence of the ALG1 gene product, we detected seven related human EST clones.
T5 488-616 Sentence denotes On searching the EST database with the amino acid sequence of the ALG1 gene product, we detected seven related human EST clones.
TextSentencer_T6 617-777 Sentence denotes A human fetal brain cDNA library was screened by PCR using gene-specific primers based on the EST nucleotide sequences and a 430 bp cDNA fragment was amplified.
T6 617-777 Sentence denotes A human fetal brain cDNA library was screened by PCR using gene-specific primers based on the EST nucleotide sequences and a 430 bp cDNA fragment was amplified.
T6 617-777 Sentence denotes A human fetal brain cDNA library was screened by PCR using gene-specific primers based on the EST nucleotide sequences and a 430 bp cDNA fragment was amplified.
TextSentencer_T7 778-899 Sentence denotes The cDNA library was rescreened with this 430 bp cDNA, and two cDNA clones (HR1-3 and HR1-4) were isolated and sequenced.
T7 778-899 Sentence denotes The cDNA library was rescreened with this 430 bp cDNA, and two cDNA clones (HR1-3 and HR1-4) were isolated and sequenced.
T7 778-899 Sentence denotes The cDNA library was rescreened with this 430 bp cDNA, and two cDNA clones (HR1-3 and HR1-4) were isolated and sequenced.
TextSentencer_T8 900-1053 Sentence denotes On a homology search of the EST database with the nucleotide sequence of HR1-3, we detected a novel human EST clone, AA675921 (GenBank accession number).
T8 900-1053 Sentence denotes On a homology search of the EST database with the nucleotide sequence of HR1-3, we detected a novel human EST clone, AA675921 (GenBank accession number).
T8 900-1053 Sentence denotes On a homology search of the EST database with the nucleotide sequence of HR1-3, we detected a novel human EST clone, AA675921 (GenBank accession number).
TextSentencer_T9 1054-1217 Sentence denotes Based on the nucleotide sequences of AA675921 and HR1-4, we designed gene-specific PCR primers, which allowed to amplify a 1.8 kb cDNA from human fetal brain cDNA.
T9 1054-1217 Sentence denotes Based on the nucleotide sequences of AA675921 and HR1-4, we designed gene-specific PCR primers, which allowed to amplify a 1.8 kb cDNA from human fetal brain cDNA.
T9 1054-1217 Sentence denotes Based on the nucleotide sequences of AA675921 and HR1-4, we designed gene-specific PCR primers, which allowed to amplify a 1.8 kb cDNA from human fetal brain cDNA.
TextSentencer_T10 1218-1305 Sentence denotes This cDNA was cloned and shown to contain an ORF encoding a protein of 464 amino acids.
T10 1218-1305 Sentence denotes This cDNA was cloned and shown to contain an ORF encoding a protein of 464 amino acids.
T10 1218-1305 Sentence denotes This cDNA was cloned and shown to contain an ORF encoding a protein of 464 amino acids.
TextSentencer_T11 1306-1339 Sentence denotes We designated this ORF as Hmat-1.
T11 1306-1339 Sentence denotes We designated this ORF as Hmat-1.
T11 1306-1339 Sentence denotes We designated this ORF as Hmat-1.
TextSentencer_T12 1340-1483 Sentence denotes The amino acid sequence deduced from the Hmat-1 gene showed several highly conserved regions shared with the yeast and nematode MT-I sequences.
T12 1340-1483 Sentence denotes The amino acid sequence deduced from the Hmat-1 gene showed several highly conserved regions shared with the yeast and nematode MT-I sequences.
T12 1340-1483 Sentence denotes The amino acid sequence deduced from the Hmat-1 gene showed several highly conserved regions shared with the yeast and nematode MT-I sequences.
TextSentencer_T13 1484-1705 Sentence denotes Furthermore, this 1.8 kb cDNA successfully complemented the S. cerevisiae alg1-1 mutation, indicating that the Hmat-1 gene encodes the human MT-I and that the function of this enzyme was conserved between yeast and human.
T13 1484-1705 Sentence denotes Furthermore, this 1.8 kb cDNA successfully complemented the S. cerevisiae alg1-1 mutation, indicating that the Hmat-1 gene encodes the human MT-I and that the function of this enzyme was conserved between yeast and human.
T13 1484-1705 Sentence denotes Furthermore, this 1.8 kb cDNA successfully complemented the S. cerevisiae alg1-1 mutation, indicating that the Hmat-1 gene encodes the human MT-I and that the function of this enzyme was conserved between yeast and human.