| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-101 |
Sentence |
denotes |
Heparan sulfate D-glucosaminyl 3-O-sulfotransferase-3A sulfates N-unsubstituted glucosamine residues. |
| T2 |
102-263 |
Sentence |
denotes |
3-O-Sulfation of glucosamine by heparan sulfate D-glucosaminyl 3-O-sulfotransferase (3-OST-1) is the key modification in anticoagulant heparan sulfate synthesis. |
| T3 |
264-471 |
Sentence |
denotes |
However, the heparan sulfates modified by 3-OST-2 and 3-OST-3A, isoforms of 3-OST-1, do not have anticoagulant activity, although these isoforms transfer sulfate to the 3-OH position of glucosamine residues. |
| T4 |
472-579 |
Sentence |
denotes |
In this study, we characterize the substrate specificity of purified 3-OST-3A at the tetrasaccharide level. |
| T5 |
580-691 |
Sentence |
denotes |
The 3-OST-3A enzyme was purified from Sf9 cells infected with recombinant baculovirus containing 3-OST-3A cDNA. |
| T6 |
692-825 |
Sentence |
denotes |
Two 3-OST-3A-modified tetrasaccharides were purified from the 3-O-(35)S-sulfated heparan sulfate that was digested by heparin lyases. |
| T7 |
826-984 |
Sentence |
denotes |
These tetrasaccharides were analyzed using nitrous acid and enzymatic degradation combined with matrix-assisted laser desorption/ionization-mass spectrometry. |
| T8 |
985-1149 |
Sentence |
denotes |
Two novel tetrasaccharides were discovered with proposed structures of DeltaUA2S-GlcNS-IdoUA2S-[(35)S]GlcNH(2)3S and DeltaUA2S-GlcNS-IdoUA2S-[3-(35)S]GlcNH(2)3S6S . |
| T9 |
1150-1329 |
Sentence |
denotes |
The results demonstrate that 3-OST-3A sulfates N-unsubstituted glucosamine residues, and the 3-OST-3A modification sites are probably located in defined oligosaccharide sequences. |
| T10 |
1330-1445 |
Sentence |
denotes |
Our study suggests that oligosaccharides with N-unsubstituted glucosamine are precursors for sulfation by 3-OST-3A. |
| T11 |
1446-1633 |
Sentence |
denotes |
The intriguing linkage between N-unsubstituted glucosamine and the 3-O-sulfation by 3-OST-3A may provide a clue to the potential biological functions of 3-OST-3A-modified heparan sulfate. |
