| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-180 |
Sentence |
denotes |
Pit-1 binding sites at the somatotrope-specific DNase I hypersensitive sites I, II of the human growth hormone locus control region are essential for in vivo hGH-N gene activation. |
| T2 |
181-261 |
Sentence |
denotes |
The human growth hormone gene cluster is composed of five closely related genes. |
| T3 |
262-391 |
Sentence |
denotes |
The 5'-most gene in the cluster, hGH-N, is expressed exclusively in somatotropes and lactosomatotropes of the anterior pituitary. |
| T4 |
392-765 |
Sentence |
denotes |
Although the hGH-N promoter contains functional binding sites for multiple transcription factors, including Sp1, Zn-15, and Pit-1, predictable and developmentally appropriate expression of hGH-N transgenes in the mouse pituitary requires the presence of a previously characterized locus control region (LCR) composed of multiple chromatin DNase I hypersensitive sites (HS). |
| T5 |
766-938 |
Sentence |
denotes |
LCR determinant(s) necessary for hGH-N transgene activation are largely conferred by two closely spaced HS (HS I,II) located 14.5 kilobase pairs upstream of the hGH-N gene. |
| T6 |
939-1069 |
Sentence |
denotes |
The region sufficient to mediate this activity has recently been sublocalized to a 404-base pair segment of HS I,II (F14 segment). |
| T7 |
1070-1205 |
Sentence |
denotes |
In the present study, we identified multiple binding sites for the pituitary POU domain transcription factor Pit-1 within this segment. |
| T8 |
1206-1380 |
Sentence |
denotes |
Using a transgenic founder assay, these sites were shown to be required for high level, position-independent, and somatotrope-specific expression of a linked hGH-N transgene. |
| T9 |
1381-1576 |
Sentence |
denotes |
Because the Pit-1 sites in the hGH-N gene promoter are insufficient for such gene activation in vivo, these data suggested a unique chromatin-mediated developmental role for Pit-1 in the hGH LCR. |
