| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-126 |
Sentence |
denotes |
The influence of endoproteolytic processing of familial Alzheimer's disease presenilin 2 on abeta42 amyloid peptide formation. |
| T2 |
127-295 |
Sentence |
denotes |
Mutant presenilins (PS) contribute to the pathogenesis of familial Alzheimer's disease (FAD) by enhancing the production of Abeta42 from beta-amyloid precursor protein. |
| T3 |
296-423 |
Sentence |
denotes |
Presenilins are endoproteolytically processed to N-terminal and C-terminal fragments, which together form a stable 1:1 complex. |
| T4 |
424-550 |
Sentence |
denotes |
We have mapped the cleavage site in the PS2 protein by direct sequencing of its C-terminal fragment isolated from mouse liver. |
| T5 |
551-747 |
Sentence |
denotes |
Three different N-terminal residues were identified starting at Val-299, Thr-301, and Leu-307 that correspond closely to the previously described N termini of the C-terminal fragment of human PS1. |
| T6 |
748-966 |
Sentence |
denotes |
Mutational analysis of the PS2 cleavage site indicates that the principal endoproteolytic cleavage occurs at residues Met-298/Val-299 and that the N terminus is subsequently modified by secondary proteolytic cleavages. |
| T7 |
967-1106 |
Sentence |
denotes |
We have generated cleavage defective PS2 constructs, which accumulate exclusively as full-length polypeptides in transfected Neuro2a cells. |
| T8 |
1107-1305 |
Sentence |
denotes |
Functional analysis of such cleavage defective PS2 carrying the FAD mutation Asn-141 --> Ile showed that its Abeta42 producing activity was strongly reduced compared with cleavage-competent FAD PS2. |
| T9 |
1306-1432 |
Sentence |
denotes |
In contrast, cleavage defective PS2 was active in rescuing the egg-laying defect of a sel-12 mutant in Caenorhabditis elegans. |
| T10 |
1433-1591 |
Sentence |
denotes |
We conclude that PS2 endoproteolytic cleavage is not an absolute requirement for its activities but may rather selectively enhance or stabilize its functions. |
