| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-150 |
Sentence |
denotes |
Pig-n, a mammalian homologue of yeast Mcd4p, is involved in transferring phosphoethanolamine to the first mannose of the glycosylphosphatidylinositol. |
| T2 |
151-308 |
Sentence |
denotes |
Many cell surface proteins are anchored to the membrane via a glycosylphosphatidylinositol (GPI) moiety, which is attached to the C terminus of the proteins. |
| T3 |
309-406 |
Sentence |
denotes |
The core of the GPI anchor is conserved in all eukaryotes but is modified by various side chains. |
| T4 |
407-586 |
Sentence |
denotes |
We cloned a mouse phosphatidylinositol glycan-class N (Pig-n) gene that encodes a 931amino acid protein expressed in the endoplasmic reticulum, which is homologous to yeast Mcd4p. |
| T5 |
587-641 |
Sentence |
denotes |
We disrupted the gene in F9 embryonal carcinoma cells. |
| T6 |
642-751 |
Sentence |
denotes |
In the Pig-n knockout cells, the first mannose in the GPI precursors was not modified by phosphoethanolamine. |
| T7 |
752-879 |
Sentence |
denotes |
Nevertheless, further biosynthetic steps continued with the addition of the third mannose and the terminal phosphoethanolamine. |
| T8 |
880-1080 |
Sentence |
denotes |
The surface expression of Thy-1 was only partially affected, indicating that modification of the first mannose by phosphoethanolamine is not essential for attachment of GPI anchors in mammalian cells. |
| T9 |
1081-1289 |
Sentence |
denotes |
An inhibitor of GPI biosynthesis, YW3548/BE49385A, inhibited transfer of phosphoethanolamine to the first mannose in mammalian cells but only slightly affected the surface expression of GPI-anchored proteins. |
| T10 |
1290-1503 |
Sentence |
denotes |
Biosynthesis of GPI in the Pig-n knockout cells was not affected by YW3548/BE49385A, and yeast overexpressing MCD4 was highly resistant to YW3548/BE49385A, suggesting that Pig-n and Mcd4p are targets of this drug. |
