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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-63 Sentence denotes Expression of a novel human sialidase encoded by the NEU2 gene.
T1 0-63 Sentence denotes Expression of a novel human sialidase encoded by the NEU2 gene.
T1 0-63 Sentence denotes Expression of a novel human sialidase encoded by the NEU2 gene.
TextSentencer_T2 64-235 Sentence denotes Sialidases (E.C.3.2.1.18) belong to a group of glycohydrolytic enzymes, widely distributed in nature, which remove sialic acid residues from glycoproteins and glycolipids.
T2 64-235 Sentence denotes Sialidases (E.C.3.2.1.18) belong to a group of glycohydrolytic enzymes, widely distributed in nature, which remove sialic acid residues from glycoproteins and glycolipids.
T2 64-235 Sentence denotes Sialidases (E.C.3.2.1.18) belong to a group of glycohydrolytic enzymes, widely distributed in nature, which remove sialic acid residues from glycoproteins and glycolipids.
TextSentencer_T3 236-438 Sentence denotes All of the sialidase so far characterized at the molecular level share an Asp block, repeated three to five times in the primary structure, and an F/YRIP sequence motif which is part of the active site.
T3 236-438 Sentence denotes All of the sialidase so far characterized at the molecular level share an Asp block, repeated three to five times in the primary structure, and an F/YRIP sequence motif which is part of the active site.
T3 236-438 Sentence denotes All of the sialidase so far characterized at the molecular level share an Asp block, repeated three to five times in the primary structure, and an F/YRIP sequence motif which is part of the active site.
TextSentencer_T4 439-559 Sentence denotes Using a sequence homology-based approach, we previously identified a human gene, named NEU2, mapping to chromosome 2q37.
T4 439-559 Sentence denotes Using a sequence homology-based approach, we previously identified a human gene, named NEU2, mapping to chromosome 2q37.
T4 439-559 Sentence denotes Using a sequence homology-based approach, we previously identified a human gene, named NEU2, mapping to chromosome 2q37.
TextSentencer_T5 560-722 Sentence denotes NEU2 encoded protein is a polypeptide of 380 amino acids with two Asp block consensuses and the YRIP sequence in the amino terminal part of the primary structure.
T5 560-722 Sentence denotes NEU2 encoded protein is a polypeptide of 380 amino acids with two Asp block consensuses and the YRIP sequence in the amino terminal part of the primary structure.
T5 560-722 Sentence denotes NEU2 encoded protein is a polypeptide of 380 amino acids with two Asp block consensuses and the YRIP sequence in the amino terminal part of the primary structure.
TextSentencer_T6 723-784 Sentence denotes Here we demonstrate that NEU2 encodes a functional sialidase.
T6 723-784 Sentence denotes Here we demonstrate that NEU2 encodes a functional sialidase.
T6 723-784 Sentence denotes Here we demonstrate that NEU2 encodes a functional sialidase.
TextSentencer_T7 785-946 Sentence denotes NEU2 was expressed in COS7 cells, giving rise to a dramatic increase in the sialidase activity measured in cell extracts with the artificial substrate 4-MU-NANA.
T7 785-946 Sentence denotes NEU2 was expressed in COS7 cells, giving rise to a dramatic increase in the sialidase activity measured in cell extracts with the artificial substrate 4-MU-NANA.
T7 785-946 Sentence denotes NEU2 was expressed in COS7 cells, giving rise to a dramatic increase in the sialidase activity measured in cell extracts with the artificial substrate 4-MU-NANA.
TextSentencer_T8 947-1156 Sentence denotes Using a rabbit polyclonal antiserum, on Western blots a protein band with a molecular weight of about 42 kDa was detectable, and its cytosolic localization was demonstrated with cell fractionation experiments.
T8 947-1156 Sentence denotes Using a rabbit polyclonal antiserum, on Western blots a protein band with a molecular weight of about 42 kDa was detectable, and its cytosolic localization was demonstrated with cell fractionation experiments.
T8 947-1156 Sentence denotes Using a rabbit polyclonal antiserum, on Western blots a protein band with a molecular weight of about 42 kDa was detectable, and its cytosolic localization was demonstrated with cell fractionation experiments.
TextSentencer_T9 1157-1223 Sentence denotes These results were confirmed using immunohistochemical techniques.
T9 1157-1223 Sentence denotes These results were confirmed using immunohistochemical techniques.
T9 1157-1223 Sentence denotes These results were confirmed using immunohistochemical techniques.
TextSentencer_T10 1224-1304 Sentence denotes NEU2 expression in E.coli cells allowed purification of the recombinant protein.
T10 1224-1304 Sentence denotes NEU2 expression in E.coli cells allowed purification of the recombinant protein.
T10 1224-1304 Sentence denotes NEU2 expression in E.coli cells allowed purification of the recombinant protein.
TextSentencer_T11 1305-1457 Sentence denotes As already observed in the enzyme expressed in COS7 cells, NEU2 pH optimum corresponds to 5.6 and the polypeptide showed a K(m)for 4-MU-NANA of 0.07 mM.
T11 1305-1457 Sentence denotes As already observed in the enzyme expressed in COS7 cells, NEU2 pH optimum corresponds to 5.6 and the polypeptide showed a K(m)for 4-MU-NANA of 0.07 mM.
T11 1305-1457 Sentence denotes As already observed in the enzyme expressed in COS7 cells, NEU2 pH optimum corresponds to 5.6 and the polypeptide showed a K(m)for 4-MU-NANA of 0.07 mM.
TextSentencer_T12 1458-1692 Sentence denotes In addition, based on the detectable similarities between the NEU2 amino acid sequence and bacterial sialidases, a prediction of the three-dimensional structure of the enzyme was carried out using a protein homology modeling approach.
T12 1458-1692 Sentence denotes In addition, based on the detectable similarities between the NEU2 amino acid sequence and bacterial sialidases, a prediction of the three-dimensional structure of the enzyme was carried out using a protein homology modeling approach.
T12 1458-1692 Sentence denotes In addition, based on the detectable similarities between the NEU2 amino acid sequence and bacterial sialidases, a prediction of the three-dimensional structure of the enzyme was carried out using a protein homology modeling approach.