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PubMed:10506200 JSONTXT 24 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-55 Sentence denotes Characterization of the human Forssman synthetase gene.
T2 56-141 Sentence denotes An evolving association between glycolipid synthesis and host-microbial interactions.
T3 142-266 Sentence denotes Differences in glycolipid expression between species contribute to the tropism of many infectious pathogens for their hosts.
T4 267-664 Sentence denotes For example, we demonstrate that cultured human and monkey urinary epithelial cells fail to bind a canine Escherichia coli uropathogenic isolate; however, transfection of these cells with the canine Forssman synthetase (FS) cDNA enables abundant adherence by the same pathogen, indicating that addition of a single sugar residue to a glycolipid receptor has marked effects on microbial attachment.
T5 665-811 Sentence denotes Given the contribution of glycolipids to host-microbial interactions, we sought to determine why human tissues do not express Forssman glycolipid.
T6 812-913 Sentence denotes Query of the GenBank(TM) data base yielded a human sequence with high identity to the canine FS cDNA.
T7 914-1045 Sentence denotes Reverse transcription polymerase chain reaction and Northern blotting demonstrated the presence of FS mRNA in all tissues examined.
T8 1046-1207 Sentence denotes A human FS cDNA was characterized, revealing identities with the canine FS gene of 86 and 83% at the nucleotide and predicted amino acid sequences, respectively.
T9 1208-1339 Sentence denotes In contrast to the canine FS cDNA, transfection of COS-1 cells with the human FS cDNA resulted in no detectable FS enzyme activity.
T10 1340-1468 Sentence denotes These results suggest that variability in glycolipid synthesis between species is an important determinant of microbial tropism.
T11 1469-1590 Sentence denotes Evolutionary pressure from pathogenic organisms may have contributed to diversity in glycolipid expression among species.