> top > projects > sentences > docs > PubMed:10473568 > annotations
sentences Home  

PubMed:10473568 JSONTXT 25 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-99 Sentence denotes Cloning and expression of a proteoglycan UDP-galactose:beta-xylose beta1,4-galactosyltransferase I.
T2 100-170 Sentence denotes A seventh member of the human beta4-galactosyltransferase gene family.
T3 171-310 Sentence denotes A seventh member of the human beta4-galactosyltransferase family, beta4Gal-T7, was identified by BLAST analysis of expressed sequence tags.
T4 311-590 Sentence denotes The coding region of beta4Gal-T7 depicts a type II transmembrane protein with sequence similarity to beta4-galactosyltransferases, but the sequence was distinct in known motifs and did not contain the cysteine residues conserved in the other six members of the beta4Gal-T family.
T5 591-671 Sentence denotes The genomic organization of beta4Gal-T7 was different from previous beta4Gal-Ts.
T6 672-848 Sentence denotes Expression of beta4Gal-T7 in insect cells showed that the gene product had beta1,4-galactosyltransferase activity with beta-xylosides, and the linkage formed was Galbeta1-4Xyl.
T7 849-1088 Sentence denotes Thus, beta4Gal-T7 represents galactosyltransferase I enzyme (xylosylprotein beta1, 4-galactosyltransferase; EC 2.4.1.133), which attaches the first galactose in the proteoglycan linkage region GlcAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser.
T8 1089-1363 Sentence denotes Sequence analysis of beta4Gal-T7 from a fibroblast cell line of a patient with a progeroid syndrome and signs of the Ehlers-Danlos syndrome, previously shown to exhibit reduced galactosyltransferase I activity (Quentin, E., Gladen, A., Rodén, L., and Kresse, H. (1990) Proc.
T9 1364-1369 Sentence denotes Natl.
T10 1370-1375 Sentence denotes Acad.
T11 1376-1380 Sentence denotes Sci.
T12 1381-1383 Sentence denotes U.
T13 1384-1386 Sentence denotes S.
T14 1387-1389 Sentence denotes A.
T15 1390-1725 Sentence denotes 87, 1342-1346), revealed two inherited allelic variants, beta4Gal-T7(186D) and beta4Gal-T7(206P), each with a single missense substitution in the putative catalytic domain of the enzyme. beta4Gal-T7(186D) exhibited a 4-fold elevated K(m) for the donor substrate, whereas essentially no activity was demonstrated with beta4Gal-T7(206P).
T16 1726-1911 Sentence denotes Molecular cloning of beta4Gal-T7 should facilitate general studies of its pathogenic role in progeroid syndromes and connective tissue disorders with affected proteoglycan biosynthesis.