| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-135 |
Sentence |
denotes |
AML1 (CBFalpha2) cooperates with B cell-specific activating protein (BSAP/PAX5) in activation of the B cell-specific BLK gene promoter. |
| T2 |
136-333 |
Sentence |
denotes |
AML1 plays a critical role during hematopoiesis and chromosomal translocations involving AML1 are commonly associated with different forms of leukemia, including pre-B acute lymphoblastic leukemia. |
| T3 |
334-603 |
Sentence |
denotes |
To understand the function of AML1 during B cell differentiation, we analyzed regulatory regions of B cell-specific genes for potential AML1-binding sites and have identified a putative AML1-binding site in the promoter of the B cell-specific tyrosine kinase gene, blk. |
| T4 |
604-799 |
Sentence |
denotes |
Gel mobility shift assays and transient transfection assays demonstrate that AML1 binds specifically to this site in the blk promoter and this binding site is important for blk promoter activity. |
| T5 |
800-990 |
Sentence |
denotes |
Furthermore, in vitro binding analysis revealed that the AML1 runt DNA-binding domain physically interacts with the paired DNA-binding domain of BSAP, a B cell-specific transcription factor. |
| T6 |
991-1085 |
Sentence |
denotes |
BSAP has been shown previously to be important for B cell-specific regulation of the blk gene. |
| T7 |
1086-1283 |
Sentence |
denotes |
Physical interaction of AML1 with BSAP correlates with functional cooperativity in transfection studies where AML1 and BSAP synergistically activate blk promoter transcription by more than 50-fold. |
| T8 |
1284-1466 |
Sentence |
denotes |
These results demonstrate physical and functional interactions between AML1 and BSAP and suggest that AML1 is an important factor for regulating a critical B cell-specific gene, blk. |
