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PubMed:10455130 JSONTXT 40 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-165 Sentence denotes Expression of core 2 beta-1,6-N-acetylglucosaminyltransferase in a human pancreatic cancer cell line results in altered expression of MUC1 tumor-associated epitopes.
T2 166-342 Sentence denotes Many tumor-associated epitopes possess carbohydrate as a key component, and thus changes in the activity of glycosyltransferases could play a role in generating these epitopes.
T3 343-576 Sentence denotes In this report we describe the stable transfection of a human pancreatic adenocarcinoma cell line, Panc1-MUC1, with the cDNA for mucin core 2 GlcNAc-transferase (C2GnT), which creates the core 2 beta-1,6 branch in mucin-type glycans.
T4 577-662 Sentence denotes These cells lack endogenous C2GnT activity but express a recombinant human MUC1 cDNA.
T5 663-839 Sentence denotes C2GnT-transfected clones expressing different levels of C2GnT were characterized using monoclonal antibodies CC49, CSLEX-1, and SM-3, which recognize tumor-associated epitopes.
T6 840-1017 Sentence denotes Increased C2GnT expression led to greatly diminished expression of the CC49 epitope, which we identified as NeuAcalpha2,6(Galbeta1,3)GalNAcalpha-Ser/Thr in the Panc1-MUC1 cells.
T7 1018-1172 Sentence denotes This was accompanied by the emergence of the CSLEX-1 epitope, sialyl Lewis x (NeuAcalpha2,3Galbeta1,4(Fucalpha1,3)GlcNAc-R), an important selectin ligand.
T8 1173-1248 Sentence denotes Despite this, however, the C2GnT transfectants could not bind to selectins.
T9 1249-1456 Sentence denotes Increased C2GnT expression also led to masking of the SM-3 peptide epitope, which persisted after the removal of sialic acid, further suggesting greater complexity of the core 2-associated O-glycans on MUC1.
T10 1457-1586 Sentence denotes The results of this study suggest that C2GnT could play a regulatory role in the expression of certain tumor-associated epitopes.