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PubMed:10438502 JSONTXT 23 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-146 Sentence denotes In vitro random mutagenesis of the D1 protein of the photosystem II reaction center confers phototolerance on the cyanobacterium Synechocystis sp.
T2 147-156 Sentence denotes PCC 6803.
T3 157-294 Sentence denotes The D1 protein of the photosystem II reaction center is thought to be the most light-sensitive component of the photosynthetic machinery.
T4 295-501 Sentence denotes To understand the mechanisms underlying the light sensitivity of D1, we performed in vitro random mutagenesis of the psbA gene that codes for D1, transformed the unicellular cyanobacterium Synechocystis sp.
T5 502-615 Sentence denotes PCC 6803 with mutated psbA, and selected phototolerant transformants that did not bleach in high intensity light.
T6 616-831 Sentence denotes A region of psbA2 coding for 178 amino acids of the carboxyl-terminal portion of the peptide was subjected to random mutagenesis by low fidelity polymerase chain reaction amplification or by hydroxylamine treatment.
T7 832-907 Sentence denotes This region contains the binding sites for Q(B), D2 (through Fe), and P680.
T8 908-1088 Sentence denotes Eighteen phototolerant mutants with single and multiple amino acid substitutions were selected from a half million transformants exposed to white light at 320 micromol m(-2) s(-1).
T9 1089-1179 Sentence denotes A strain transformed with non-mutagenized psbA2 became bleached under the same conditions.
T10 1180-1339 Sentence denotes Site-directed mutagenesis has confirmed that one or more substitutions of amino acids at residues 234, 254, 260, 267, 322, 326, and 328 confers phototolerance.
T11 1340-1424 Sentence denotes The rate of degradation of D1 protein was not appreciably affected by the mutations.
T12 1425-1597 Sentence denotes Reduced bleaching of mutant cyanobacterial cells may result from continued buildup of photosynthetic pigment systems caused by changes in redox signals originating from D1.