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Id Subject Object Predicate Lexical cue
T1 0-67 Sentence denotes Effect of alternative glycosylation on insulin receptor processing.
T2 68-189 Sentence denotes The mature insulin receptor is a cell surface heterotetrameric glycoprotein composed of two alpha- and two beta-subunits.
T3 190-373 Sentence denotes In 3T3-L1 adipocytes as in other cell types, the receptor is synthesized as a single polypeptide consisting of uncleaved alpha- and beta-subunits, migrating as a 190-kDa glycoprotein.
T4 374-532 Sentence denotes To examine the importance of N-linked glycosylation on insulin receptor processing, we have used glucose deprivation as a tool to alter protein glycosylation.
T5 533-741 Sentence denotes Western blot analysis shows that glucose deprivation led to a time-dependent accumulation of an alternative proreceptor of 170 kDa in a subcellular fraction consistent with endoplasmic reticulum localization.
T6 742-874 Sentence denotes Co-precipitation assays provide evidence that the alternative proreceptor bound GRP78, an endoplasmic reticulum molecular chaperone.
T7 875-976 Sentence denotes N-Glycosidase F treatment shows that the alternative proreceptor contained N-linked oligosaccharides.
T8 977-1062 Sentence denotes Yet, endoglycosidase H insensitivity indicates an aberrant oligosaccharide structure.
T9 1063-1185 Sentence denotes Using pulse-chase methodology, we show that the synthetic rate was similar between the normal and alternative proreceptor.
T10 1186-1368 Sentence denotes However, the normal proreceptor was processed into alpha- and beta-subunits (t((1)/(2)) = 1.3 +/- 0.6 h), while the alternative proreceptor was degraded (t((1)/(2)) = 5.1 +/- 0.6 h).
T11 1369-1551 Sentence denotes Upon refeeding cells that were initially deprived of glucose, the alternative proreceptor was processed to a higher molecular weight form and gained sensitivity to endoglycosidase H.
T12 1552-1716 Sentence denotes This "intermediate" form of the proreceptor was also degraded, although a small fraction escaped degradation, resulting in cleavage to the alpha- and beta-subunits.
T13 1717-1977 Sentence denotes These data provide evidence for the first time that glucose deprivation leads to the accumulation of an alternative proreceptor, which can be post-translationally glycosylated with the readdition of glucose inducing both accelerated degradation and maturation.