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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-63 Sentence denotes Mass spectrometric analysis of nitric oxide-modified caspase-3.
T1 0-63 Sentence denotes Mass spectrometric analysis of nitric oxide-modified caspase-3.
TextSentencer_T2 64-135 Sentence denotes Caspases are a family of cysteine proteases activated during apoptosis.
T2 64-135 Sentence denotes Caspases are a family of cysteine proteases activated during apoptosis.
TextSentencer_T3 136-249 Sentence denotes Modification of caspases by nitric oxide and its relevance during apoptosis is currently a controversial subject.
T3 136-249 Sentence denotes Modification of caspases by nitric oxide and its relevance during apoptosis is currently a controversial subject.
TextSentencer_T4 250-332 Sentence denotes In this study we analyzed the S-nitrosated form of caspase-3 at a molecular level.
T4 250-332 Sentence denotes In this study we analyzed the S-nitrosated form of caspase-3 at a molecular level.
TextSentencer_T5 333-489 Sentence denotes By using electrospray ionization-mass spectrometry, we detected poly-S-nitrosation of caspase-3 with an average of about 2 molecules of NO bound per enzyme.
T5 333-489 Sentence denotes By using electrospray ionization-mass spectrometry, we detected poly-S-nitrosation of caspase-3 with an average of about 2 molecules of NO bound per enzyme.
TextSentencer_T6 490-611 Sentence denotes Although NO treatment completely inhibited enzyme activity, S-nitrosation was not restricted to the active site cysteine.
T6 490-611 Sentence denotes Although NO treatment completely inhibited enzyme activity, S-nitrosation was not restricted to the active site cysteine.
TextSentencer_T7 612-775 Sentence denotes Rather, we detected multiple relative mass increases of 30 +/- 1 Da in both the p12 and p17 subunits of caspase-3, corresponding to single to triple S-nitrosation.
T7 612-775 Sentence denotes Rather, we detected multiple relative mass increases of 30 +/- 1 Da in both the p12 and p17 subunits of caspase-3, corresponding to single to triple S-nitrosation.
TextSentencer_T8 776-886 Sentence denotes The stability of these S-nitrosations differed in physiologically relevant concentrations of 5 mM glutathione.
T8 776-886 Sentence denotes The stability of these S-nitrosations differed in physiologically relevant concentrations of 5 mM glutathione.
TextSentencer_T9 887-1092 Sentence denotes Whereas all S-nitroso bonds in the p12 subunit were cleaved with release of NO and partial formation of protein-mixed disulfides with glutathione, a single S-nitrosation in the p17 subunit remained stable.
T9 887-1092 Sentence denotes Whereas all S-nitroso bonds in the p12 subunit were cleaved with release of NO and partial formation of protein-mixed disulfides with glutathione, a single S-nitrosation in the p17 subunit remained stable.
TextSentencer_T10 1093-1355 Sentence denotes Since this S-nitrosation was not observed in a mutant form of caspase-3 lacking the active site cysteine, we conclude that NO nitrosates the active site cysteine of caspase-3 and that this modification is notably inert to fast trans-nitrosation with glutathione.
T10 1093-1355 Sentence denotes Since this S-nitrosation was not observed in a mutant form of caspase-3 lacking the active site cysteine, we conclude that NO nitrosates the active site cysteine of caspase-3 and that this modification is notably inert to fast trans-nitrosation with glutathione.
TextSentencer_T11 1356-1525 Sentence denotes Furthermore, we provide evidence that treatment of caspase-3 with NO can lead to mixed disulfide formation with glutathione, demonstrating the oxidative character of NO.
T11 1356-1525 Sentence denotes Furthermore, we provide evidence that treatment of caspase-3 with NO can lead to mixed disulfide formation with glutathione, demonstrating the oxidative character of NO.