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PubMed:10406843 JSONTXT 73 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-135 Sentence denotes Molecular cloning and characterization of cDNA coding for beta1, 2N-acetylglucosaminyltransferase I (GlcNAc-TI) from Nicotiana tabacum.
T1 0-135 Sentence denotes Molecular cloning and characterization of cDNA coding for beta1, 2N-acetylglucosaminyltransferase I (GlcNAc-TI) from Nicotiana tabacum.
TextSentencer_T2 136-401 Sentence denotes In plants as well as in animals beta1, 2N-acetylglucosaminyltransferase I (GlcNAc-TI) is a Golgi resident enzyme that catalyzes an essential step in the biosynthetic pathway leading from oligomannosidic N-glycans to complex or hybrid type N-linked oligosaccharides.
T2 136-401 Sentence denotes In plants as well as in animals beta1, 2N-acetylglucosaminyltransferase I (GlcNAc-TI) is a Golgi resident enzyme that catalyzes an essential step in the biosynthetic pathway leading from oligomannosidic N-glycans to complex or hybrid type N-linked oligosaccharides.
TextSentencer_T3 402-574 Sentence denotes Employing degenerated primers deduced from known GlcNAc-TI genes from animals, we were able to identify the cDNA coding for GlcNAc-TI from a Nicotiana tabacum cDNA library.
T3 402-574 Sentence denotes Employing degenerated primers deduced from known GlcNAc-TI genes from animals, we were able to identify the cDNA coding for GlcNAc-TI from a Nicotiana tabacum cDNA library.
TextSentencer_T4 575-701 Sentence denotes The complete nucleotide sequence revealed a 1338 base pair open reading frame that codes for a polypeptide of 446 amino acids.
T4 575-701 Sentence denotes The complete nucleotide sequence revealed a 1338 base pair open reading frame that codes for a polypeptide of 446 amino acids.
TextSentencer_T5 702-909 Sentence denotes Comparison of the deduced amino acid sequence with that of already known GlcNAc-TI polypeptides revealed no similarity of the tobacco clone within the putative cytoplasmatic, transmembrane, and stem regions.
T5 702-909 Sentence denotes Comparison of the deduced amino acid sequence with that of already known GlcNAc-TI polypeptides revealed no similarity of the tobacco clone within the putative cytoplasmatic, transmembrane, and stem regions.
TextSentencer_T6 910-1060 Sentence denotes However, 40% sequence similarity was found within the putative C-terminal catalytic domain containing conserved single amino acids and peptide motifs.
T6 910-1060 Sentence denotes However, 40% sequence similarity was found within the putative C-terminal catalytic domain containing conserved single amino acids and peptide motifs.
TextSentencer_T7 1061-1219 Sentence denotes The predicted domain structure of the tobacco polypeptide is typical for type II transmembrane proteins and comparable to known GlcNAc-TI from animal species.
T7 1061-1219 Sentence denotes The predicted domain structure of the tobacco polypeptide is typical for type II transmembrane proteins and comparable to known GlcNAc-TI from animal species.
TextSentencer_T8 1220-1382 Sentence denotes In order to confirm enzyme activity a truncated form of the protein containing the putative catalytic domain was expressed using a baculovirus/insect cell system.
T8 1220-1382 Sentence denotes In order to confirm enzyme activity a truncated form of the protein containing the putative catalytic domain was expressed using a baculovirus/insect cell system.
TextSentencer_T9 1383-1517 Sentence denotes Using pyridylaminated Man(5)- or Man(3)GlcNAc(2)as acceptor substrates and HPLC analysis of the products GlcNAc-TI activity was shown.
T9 1383-1517 Sentence denotes Using pyridylaminated Man(5)- or Man(3)GlcNAc(2)as acceptor substrates and HPLC analysis of the products GlcNAc-TI activity was shown.
TextSentencer_T10 1518-1609 Sentence denotes This demonstrates that the C-terminal region of the protein comprises the catalytic domain.
T10 1518-1609 Sentence denotes This demonstrates that the C-terminal region of the protein comprises the catalytic domain.
TextSentencer_T11 1610-1683 Sentence denotes Expression of GlcNAc-TI mRNA in tobacco leaves was detected using RT-PCR.
T11 1610-1683 Sentence denotes Expression of GlcNAc-TI mRNA in tobacco leaves was detected using RT-PCR.
TextSentencer_T12 1684-1839 Sentence denotes Southern blot analysis gave two hybridization signals of the gene in the amphidiploid genomes of the two investigated species N. tabacum and N.benthamiana.
T12 1684-1839 Sentence denotes Southern blot analysis gave two hybridization signals of the gene in the amphidiploid genomes of the two investigated species N. tabacum and N.benthamiana.