| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-89 |
Sentence |
denotes |
Ubc9 interacts with the androgen receptor and activates receptor-dependent transcription. |
| T2 |
90-281 |
Sentence |
denotes |
Ubc9, a homologue of the class E2 ubiquitin-conjugating enzymes, has recently been shown to catalyze conjugation of a small ubiquitin-like molecule-1 (SUMO-1) to a variety of target proteins. |
| T3 |
282-484 |
Sentence |
denotes |
SUMO-1 modifications have been implicated in the targeting of proteins to the nuclear envelope and certain intranuclear structures and in converting proteins resistant to ubiquitin-mediated degradation. |
| T4 |
485-649 |
Sentence |
denotes |
In the present work, we find that Ubc9 interacts with the androgen receptor (AR), a member of the steroid receptor family of ligand-activated transcription factors. |
| T5 |
650-771 |
Sentence |
denotes |
In transiently transfected COS-1 cells, AR-dependent but not basal transcription is enhanced by the coexpression of Ubc9. |
| T6 |
772-934 |
Sentence |
denotes |
The N-terminal half of the AR hinge region containing the C-terminal part of the bipartite nuclear localization signal is essential for the interaction with Ubc9. |
| T7 |
935-1162 |
Sentence |
denotes |
Deletion of this part of the nuclear localization signal, which does not completely prevent the transfer of AR to the nucleus, abolishes the AR-Ubc9 interaction and attenuates the transcriptional response to cotransfected Ubc9. |
| T8 |
1163-1513 |
Sentence |
denotes |
The C93S substitution of Ubc9, which prevents SUMO-1 conjugation by abrogating the formation of a thiolester bond between SUMO-1 and Ubc9, does not influence the capability of Ubc9 to stimulate AR-dependent transactivation, implying that Ubc9 is able to act as an AR coregulator in a fashion independent of its ability to catalyze SUMO-1 conjugation. |
