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PubMed:10383450 JSONTXT 37 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-100 Sentence denotes Intracellular accumulation of secreted proteoglycans inhibits cationic lipid-mediated gene transfer.
T2 101-150 Sentence denotes Co-transfer of glycosaminoglycans to the nucleus.
T3 151-253 Sentence denotes Molecules secreted by potential target cells may interfere with cationic lipid-mediated gene transfer.
T4 254-344 Sentence denotes This has been studied using human lung fibroblasts and human epidermoid lung cancer cells.
T5 345-517 Sentence denotes Secreted cell medium components caused a substantial decrease both in the uptake of cationic lipid-DNA complexes (2-4-fold) and in reporter gene expression (100-1000-fold).
T6 518-667 Sentence denotes Metabolic labeling of the cell medium showed that especially [35S]sulfate-labeled macromolecules competed with DNA for binding to the cationic lipid.
T7 668-793 Sentence denotes Release of DNA from the cationic lipid by cell medium components was demonstrated by an ethidium bromide intercalation assay.
T8 794-892 Sentence denotes In the presence of the cationic lipid, the secreted macromolecules were internalized by the cells.
T9 893-1155 Sentence denotes By enzymatic digestions, it was shown that the competing macromolecules consist of chondroitin/dermatan sulfate and heparan sulfate proteoglycans and that the effects on transfection were mediated by the polyanionic glycosaminoglycan portion of the proteoglycan.
T10 1156-1295 Sentence denotes Accordingly, pretreatment of cell medium with the polycationic peptide protamine sulfate abrogated the inhibitory effects on gene transfer.
T11 1296-1438 Sentence denotes Fluorescence microscopy studies revealed that heparan sulfate, internalized as a complex with cationic lipids, accumulated in the cell nuclei.
T12 1439-1603 Sentence denotes These results support the view that the lack of specificity of this type of gene transfer vehicle is a major hindrance to efficient and safe in vivo administration.