| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-105 |
Sentence |
denotes |
SLP-76 and Vav function in separate, but overlapping pathways to augment interleukin-2 promoter activity. |
| T2 |
106-216 |
Sentence |
denotes |
SLP-76 and Vav, two hematopoietic cell specific molecules, are critical for T cell development and activation. |
| T3 |
217-412 |
Sentence |
denotes |
Following T cell antigen receptor stimulation, SLP-76 and Vav both undergo tyrosine phosphorylation and associate with each other via the SH2 domain of Vav and phosphorylated tyrosines of SLP-76. |
| T4 |
413-518 |
Sentence |
denotes |
Furthermore, SLP-76 and Vav have a synergistic effect on interleukin (IL)-2 promoter activity in T cells. |
| T5 |
519-665 |
Sentence |
denotes |
In this report, we show that two tyrosines, Tyr-113 and Tyr-128, of SLP-76 are required for its binding to Vav, both in vitro and in intact cells. |
| T6 |
666-918 |
Sentence |
denotes |
Surprisingly, we find also that the interaction between SLP-76 and Vav is not required for their cooperation in augmenting IL-2 promoter activity, as the two molecules appear to function in different signaling pathways upstream of IL-2 gene expression. |
| T7 |
919-1072 |
Sentence |
denotes |
Overexpression of SLP-76 in the Jurkat T cell line potentiates the activities of both nuclear factor of activated T cells and AP-1 transcription factors. |
| T8 |
1073-1194 |
Sentence |
denotes |
In contrast, overexpression of Vav leads to enhanced nuclear factor of activated T cells activity without affecting AP-1. |
| T9 |
1195-1293 |
Sentence |
denotes |
Additionally, overexpression of Vav, but not SLP-76, augments CD28-induced IL-2 promoter activity. |
| T10 |
1294-1454 |
Sentence |
denotes |
These findings suggest that the synergy between SLP-76 and Vav in regulating IL-2 gene expression reflects the cooperation between different signaling pathways. |
