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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-105 Sentence denotes Characterization of the O-linked oligosaccharide structures on P-selectin glycoprotein ligand-1 (PSGL-1).
T1 0-105 Sentence denotes Characterization of the O-linked oligosaccharide structures on P-selectin glycoprotein ligand-1 (PSGL-1).
TextSentencer_T2 106-379 Sentence denotes P-selectin glycoprotein ligand-1, PSGL-1, a specific ligand for P-, E-, and L-selectin, was isolated from in vivo [3H]-glucosamine labeled HL-60 cells by a combination of wheat germ agglutinin and platelet P-selectin- or E-selectin receptor globulin-agarose chromatography.
T2 106-379 Sentence denotes P-selectin glycoprotein ligand-1, PSGL-1, a specific ligand for P-, E-, and L-selectin, was isolated from in vivo [3H]-glucosamine labeled HL-60 cells by a combination of wheat germ agglutinin and platelet P-selectin- or E-selectin receptor globulin-agarose chromatography.
TextSentencer_T3 380-657 Sentence denotes The O-linked oligosaccharides on the ligand were released by mild alkaline sodium borohydride treatment and analyzed by a combination of ion-exchange, size exclusion, lectin, and paper chromatography, together with specific exoglycosidase treatments and chemical modifications.
T3 380-657 Sentence denotes The O-linked oligosaccharides on the ligand were released by mild alkaline sodium borohydride treatment and analyzed by a combination of ion-exchange, size exclusion, lectin, and paper chromatography, together with specific exoglycosidase treatments and chemical modifications.
TextSentencer_T4 658-757 Sentence denotes Approximately 91% of the radioactivity released from PSGL-1 was recovered in five O-linked glycans:
T4 658-757 Sentence denotes Approximately 91% of the radioactivity released from PSGL-1 was recovered in five O-linked glycans:
TextSentencer_T5 758-936 Sentence denotes GalNAc (approximately 4% of the total structures), Galp, 3GalNAc (36%), and Galbeta, 3GalNAc substituted with one (45%), two (6%), or three (3%) N-acetyllactosamine repeat units.
T5 758-936 Sentence denotes GalNAc (approximately 4% of the total structures), Galp, 3GalNAc (36%), and Galbeta, 3GalNAc substituted with one (45%), two (6%), or three (3%) N-acetyllactosamine repeat units.
TextSentencer_T6 937-1044 Sentence denotes None of these structures contained fucose, and the majority were substituted with at least one sialic acid.
T6 937-1044 Sentence denotes None of these structures contained fucose, and the majority were substituted with at least one sialic acid.
TextSentencer_T7 1045-1112 Sentence denotes The N-acetyllactosmine-containing structures appeared to be core 2.
T7 1045-1112 Sentence denotes The N-acetyllactosmine-containing structures appeared to be core 2.
TextSentencer_T8 1113-1288 Sentence denotes The remaining 9% of the radioactivity recovered in O-linked oligosaccharides from PSGL-1, eluted in two peaks at 11.8 and 10.2 glucose units, on size-exclusion chromatography.
T8 1113-1288 Sentence denotes The remaining 9% of the radioactivity recovered in O-linked oligosaccharides from PSGL-1, eluted in two peaks at 11.8 and 10.2 glucose units, on size-exclusion chromatography.
TextSentencer_T9 1289-1589 Sentence denotes Results from lectin chromatography and chemical and enzymatic degradation experiments suggest that the major portion of the radioactivity in these peaks is associated with sialylated N-acetyllactosamine-type oligosaccharides, substituted with fucose at the penultimate residue in the nonreducing end.
T9 1289-1589 Sentence denotes Results from lectin chromatography and chemical and enzymatic degradation experiments suggest that the major portion of the radioactivity in these peaks is associated with sialylated N-acetyllactosamine-type oligosaccharides, substituted with fucose at the penultimate residue in the nonreducing end.
TextSentencer_T10 1590-1845 Sentence denotes Since both sialic acid and fucose reportedly are crucial requirements for selectin binding, these results suggest that only a minor portion, approximately 4.5%, of the O-linked oligosaccharides on PSGL-1 are involved in the interaction with the selectins.
T10 1590-1845 Sentence denotes Since both sialic acid and fucose reportedly are crucial requirements for selectin binding, these results suggest that only a minor portion, approximately 4.5%, of the O-linked oligosaccharides on PSGL-1 are involved in the interaction with the selectins.