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PubMed:10187864 JSONTXT 25 Projects

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Id Subject Object Predicate Lexical cue
T1 0-90 Sentence denotes Cloning, characterization, and chromosomal location of a novel human K+-Cl- cotransporter.
T2 91-281 Sentence denotes Differential display polymerase chain reaction has been used to isolate genes regulated in vascular endothelial cells by the angiogenic factor vascular endothelial cell growth factor (VEGF).
T3 282-501 Sentence denotes Analysis of one of the bands consistently up-regulated by VEGF led us to the identification of a cDNA from a human umbilical vein endothelial cell library that is 77% identical to the human K+-Cl- cotransporter1 (KCC1).
T4 502-577 Sentence denotes We have referred to the predicted protein as K+-Cl- cotransporter 3 (KCC3).
T5 578-827 Sentence denotes Hydrophobicity analysis of the KCC3 amino acid sequence showed an almost identical pattern to KCC1, suggesting 12 membrane-spanning segments, a large extracellular loop with potential N-glycosylation sites, and cytoplasmic N- and C-terminal regions.
T6 828-964 Sentence denotes The KCC3 mRNA was highly expressed in brain, heart, skeletal muscle, and kidney, showing a distinct pattern and size from KCC1 and KCC2.
T7 965-1160 Sentence denotes The KCC3 mRNA level in endothelial cells increased on treatment with VEGF and decreased with the proinflammatory cytokine tumor necrosis factor alpha, whereas KCC1 mRNA levels remained unchanged.
T8 1161-1316 Sentence denotes Stable overexpression of KCC3 cDNA in HEK293 cells produced a glycoprotein of approximately 150 kDa, which was reduced to 120 kDa by glycosidase digestion.
T9 1317-1515 Sentence denotes An increased initial uptake rate of 86Rb was seen in clones with high KCC3 expression, which was dependent on extracellular Cl- but not Na+ and was inhibitable by the loop diuretic agent furosemide.
T10 1516-1606 Sentence denotes The KCC3 genomic localization was shown to be 15q13 by fluorescence in situ hybridization.
T11 1607-1704 Sentence denotes Radiation hybrid analysis placed KCC3 within an area associated with juvenile myoclonic epilepsy.
T12 1705-1810 Sentence denotes These results suggest KCC3 is a new member of the KCC family that is under distinct regulation from KCC1.