| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-88 |
Sentence |
denotes |
Spi-C, a novel Ets protein that is temporally regulated during B lymphocyte development. |
| T2 |
89-287 |
Sentence |
denotes |
A novel Ets protein was isolated by yeast one-hybrid screening of a cDNA library made from lipopolysaccharide-stimulated mouse splenic B cells, using the SP6 kappa promoter kappaY element as a bait. |
| T3 |
288-417 |
Sentence |
denotes |
The novel Ets protein was most closely related to PU.1 and Spi-B within the DNA binding Ets domain and was therefore named Spi-C. |
| T4 |
418-577 |
Sentence |
denotes |
However, Spi-C may represent a novel subgroup within the Ets protein family, as it differed significantly from Spi-B and PU.1 within helix 1 of the Ets domain. |
| T5 |
578-661 |
Sentence |
denotes |
Spi-C was encoded by a single-copy gene that was mapped to chromosome 10, region C. |
| T6 |
662-882 |
Sentence |
denotes |
Spi-C interacted with DNA similarly to PU.1 as judged by methylation interference, band-shift and site selection analysis, and activated transcription of a kappaY element reporter gene upon co-transfection of HeLa cells. |
| T7 |
883-966 |
Sentence |
denotes |
Spi-C RNA was expressed in mature B lymphocytes and at lower levels in macrophages. |
| T8 |
967-1137 |
Sentence |
denotes |
Furthermore, pre-B cell and plasma cell lines were Spi-C-negative, suggesting that Spi-C might be a regulatory molecule during a specific phase of B lymphoid development. |
