| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-60 |
Sentence |
denotes |
Regulation of I-branched poly-N-acetyllactosamine synthesis. |
| T2 |
61-158 |
Sentence |
denotes |
Concerted actions by I-extension enzyme, I-branching enzyme, and beta1,4-galactosyltransferase I. |
| T3 |
159-383 |
Sentence |
denotes |
I-branched poly-N-acetyllactosamine is a unique carbohydrate composed of N-acetyllactosamine branches attached to linear poly-N-acetyllactosamine, which is synthesized by I-branching beta1, 6-N-acetylglucosaminyltransferase. |
| T4 |
384-581 |
Sentence |
denotes |
I-branched poly-N-acetyllactosamine can carry bivalent functional oligosaccharides such as sialyl Lewisx, which provide much better carbohydrate ligands than monovalent functional oligosaccharides. |
| T5 |
582-856 |
Sentence |
denotes |
In the present study, we first demonstrate that I-branching beta1, 6-N-acetylglucosaminyltransferase cloned from human PA-1 embryonic carcinoma cells transfers beta1,6-linked GlcNAc preferentially to galactosyl residues of N-acetyllactosamine close to nonreducing terminals. |
| T6 |
857-1044 |
Sentence |
denotes |
We then demonstrate that among various beta1, 4-galactosyltransferases (beta4Gal-Ts), beta4Gal-TI is most efficient in adding a galactose to linear and branched poly-N-acetyllactosamines. |
| T7 |
1045-1336 |
Sentence |
denotes |
When a beta1,6-GlcNAc branched poly-N-acetyllactosamine was incubated with a mixture of beta4Gal-TI and i-extension beta1,3-N-acetylglucosaminyltransferase, the major product was the oligosaccharide with one N-acetyllactosamine extension on the linear Galbeta1-->4GlcNAcbeta1-->3 side chain. |
| T8 |
1337-1430 |
Sentence |
denotes |
Only a minor product contained galactosylated I-branch without N-acetyllactosamine extension. |
| T9 |
1431-1710 |
Sentence |
denotes |
This finding was explained by the fact that beta4Gal-TI adds a galactose poorly to beta1,6-GlcNAc attached to linear poly-N-acetyllactosamines, while beta1, 3-N-acetylglucosaminyltransferase and beta4Gal-TI efficiently add N-acetyllactosamine to linear poly-N-acetyllactosamines. |
| T10 |
1711-1963 |
Sentence |
denotes |
Together, these results strongly suggest that galactosylation of I-branch is a rate-limiting step in I-branched poly-N-acetyllactosamine synthesis, allowing poly-N-acetyllactosamine extension mostly along the linear poly-N-acetyllactosamine side chain. |
| T11 |
1964-2171 |
Sentence |
denotes |
These findings are entirely consistent with previous findings that poly-N-acetyllactosamines in human erythrocytes, PA-1 embryonic carcinoma cells, and rabbit erythrocytes contain multiple, short I-branches. |
