| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-88 |
Sentence |
denotes |
Cloning and characterization of androgen receptor coactivator, ARA55, in human prostate. |
| T2 |
89-306 |
Sentence |
denotes |
Androgen receptor (AR) is a hormone-activated transcriptional factor that can bind to androgen response elements and that regulates the transcription of target genes via a mechanism that presumably involves cofactors. |
| T3 |
307-398 |
Sentence |
denotes |
We report here the cloning of a novel AR coactivator ARA55 using a yeast two-hybrid system. |
| T4 |
399-561 |
Sentence |
denotes |
ARA55 consists of 444 amino acids with the predicted molecular mass of 55 kDa and its sequence shows very high homology to mouse hic5, a TGF-beta1-inducible gene. |
| T5 |
562-695 |
Sentence |
denotes |
Yeast and mammalian two-hybrid systems and co-immunoprecipitation assays all prove ARA55 can bind to AR in a ligand-dependent manner. |
| T6 |
696-952 |
Sentence |
denotes |
Transient transfection assay in prostate cancer DU145 cells further demonstrates that ARA55 can enhance AR transcriptional activity in the presence of 1 nM dihydrotestosterone or its antagonists such as 100 nM 17beta-estradiol or 1 microM hydroxyflutamide. |
| T7 |
953-1072 |
Sentence |
denotes |
Our data also suggest the C-terminal half of ARA55, which includes three LIM motifs, is sufficient to interact with AR. |
| T8 |
1073-1216 |
Sentence |
denotes |
Northern blot and polymerase chain reaction quantitation showed ARA55 can be expressed differently in normal prostate and prostate tumor cells. |
| T9 |
1217-1364 |
Sentence |
denotes |
Together, our data suggests that ARA55 may play very important roles in the progression of prostate cancer by the modulation of AR transactivation. |
