| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-96 |
Sentence |
denotes |
Skeletal muscle type ryanodine receptor is involved in calcium signaling in human B lymphocytes. |
| T2 |
97-362 |
Sentence |
denotes |
The regulation of intracellular free Ca2+ concentration ([Ca2+]i) in B cells remains poorly understood and is presently explained almost solely by inositol 1,4,5-triphosphate (IP3)-mediated Ca2+ release, followed by activation of a store-operated channel mechanism. |
| T3 |
363-482 |
Sentence |
denotes |
In fact, there are reports indicating that IP3 production does not always correlate with the magnitude of Ca2+ release. |
| T4 |
483-673 |
Sentence |
denotes |
We demonstrate here that human B cells express a ryanodine receptor (RYR) that functions as a Ca2+ release channel during the B cell antigen receptor (BCR)-stimulated Ca2+ signaling process. |
| T5 |
674-874 |
Sentence |
denotes |
Immunoblotting studies showed that both human primary CD19(+) B and DAKIKI cells express a 565-kDa immunoreactive protein that is indistinguishable in molecular size and immunoreactivity from the RYR. |
| T6 |
875-1146 |
Sentence |
denotes |
Selective reverse transcription-polymerase chain reaction, restriction fragment length polymorphism, and sequencing of cloned cDNA indicated that the major isoform of the RYR expressed in primary CD19(+) B and DAKIKI cells is identical to the skeletal muscle type (RYR1). |
| T7 |
1147-1262 |
Sentence |
denotes |
Saturation analysis of [3H]ryanodine binding yielded Bmax = 150 fmol/mg of protein and Kd = 110 nM in DAKIKI cells. |
| T8 |
1263-1493 |
Sentence |
denotes |
In fluo-3-loaded CD19(+) B and DAKIKI cells, 4-chloro-m-cresol, a potent activator of Ca2+ release mediated by the ryanodine-sensitive Ca2+ release channel, induced Ca2+ release in a dose-dependent and ryanodine-sensitive fashion. |
| T9 |
1494-1613 |
Sentence |
denotes |
Furthermore, BCR-mediated Ca2+ release in CD19(+) B cells was significantly altered by 4-chloro-m-cresol and ryanodine. |
| T10 |
1614-1867 |
Sentence |
denotes |
These results indicate that RYR1 functions as a Ca2+ release channel during BCR-stimulated Ca2+ signaling and suggest that complex Ca2+ signals that control the cellular activities of B cells may be generated by cooperation of the IP3 receptor and RYR1. |
