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PubMed:10026210 JSONTXT 24 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-104 Sentence denotes A novel PDZ domain containing guanine nucleotide exchange factor links heterotrimeric G proteins to Rho.
T2 105-194 Sentence denotes Small GTP-binding proteins of the Rho family play a critical role in signal transduction.
T3 195-296 Sentence denotes However, there is still very limited information on how they are activated by cell surface receptors.
T4 297-562 Sentence denotes Here, we used a consensus sequence for Dbl domains of Rho guanine nucleotide exchange factors (GEFs) to search DNA data bases, and identified a novel human GEF for Rho-related GTPases harboring structural features indicative of its possible regulatory mechanism(s).
T5 563-802 Sentence denotes This protein contained a tandem DH/PH domain closely related to those of Rho-specific GEFs, a PDZ domain, a proline-rich domain, and an area of homology to Lsc, p115-RhoGEF, and a Drosophila RhoGEF that was termed Lsc-homology (LH) domain.
T6 803-980 Sentence denotes This novel molecule, designated PDZ-RhoGEF, activated biological and biochemical pathways specific for Rho, and activation of these pathways required an intact DH and PH domain.
T7 981-1150 Sentence denotes However, the PDZ domain was dispensable for these functions, and mutants lacking the LH domain were more active, suggesting a negative regulatory role for the LH domain.
T8 1151-1348 Sentence denotes A search for additional molecules exhibiting an LH domain revealed a limited homology with the catalytic region of a newly identified GTPase-activating protein for heterotrimeric G proteins, RGS14.
T9 1349-1468 Sentence denotes This prompted us to investigate whether PDZ-RhoGEF could interact with representative members of each G protein family.
T10 1469-1658 Sentence denotes We found that PDZ-RhoGEF was able to form, in vivo, stable complexes with two members of the Galpha12 family, Galpha12 and Galpha13, and that this interaction was mediated by the LH domain.
T11 1659-1776 Sentence denotes Furthermore, we obtained evidence to suggest that PDZ-RhoGEF mediates the activation of Rho by Galpha12 and Galpha13.
T12 1777-2105 Sentence denotes Together, these findings suggest the existence of a novel mechanism whereby the large family of cell surface receptors that transmit signals through heterotrimeric G proteins activate Rho-dependent pathways: by stimulating the activity of members of the Galpha12 family which, in turn, activate an exchange factor acting on Rho.