| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-123 |
Sentence |
denotes |
Characterization of a mutant pancreatic eIF-2alpha kinase, PEK, and co-localization with somatostatin in islet delta cells. |
| T2 |
124-316 |
Sentence |
denotes |
Phosphorylation of eukaryotic translation initiation factor-2alpha (eIF-2alpha) is one of the key steps where protein synthesis is regulated in response to changes in environmental conditions. |
| T3 |
317-529 |
Sentence |
denotes |
The phosphorylation is carried out in part by three distinct eIF-2alpha kinases including mammalian double-stranded RNA-dependent eIF-2alpha kinase (PKR) and heme-regulated inhibitor kinase (HRI), and yeast GCN2. |
| T4 |
530-714 |
Sentence |
denotes |
We report the identification and characterization of a related kinase, PEK, which shares common features with other eIF-2alpha kinases including phosphorylation of eIF-2alpha in vitro. |
| T5 |
715-791 |
Sentence |
denotes |
We show that human PEK is regulated by different mechanisms than PKR or HRI. |
| T6 |
792-1092 |
Sentence |
denotes |
In contrast to PKR or HRI, which are dependent on autophosphorylation for their kinase activity, a point mutation that replaced the conserved Lys-614 with an alanine completely abolished the eIF-2alpha kinase activity, whereas the mutant PEK was still autophosphorylated when expressed in Sf-9 cells. |
| T7 |
1093-1239 |
Sentence |
denotes |
Northern blot analysis indicates that PEK mRNA was predominantly expressed in pancreas, though low expression was also present in several tissues. |
| T8 |
1240-1443 |
Sentence |
denotes |
Consistent with the high levels of mRNA in pancreas, the PEK protein was only detected in human pancreatic islets, and the kinase co-localized with somatostatin, a pancreatic delta cell-specific hormone. |
| T9 |
1444-1580 |
Sentence |
denotes |
Thus PEK is believed to play an important role in regulating protein synthesis in the pancreatic islet, especially in islet delta cells. |
