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PubMed:10026142 JSONTXT 23 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-93 Sentence denotes Purification and identification of a novel subunit of protein serine/threonine phosphatase 4.
T2 94-265 Sentence denotes The catalytic subunit of protein serine/threonine phosphatase 4 (PP4C) has greater than 65% amino acid identity to the catalytic subunit of protein phosphatase 2A (PP2AC).
T3 266-363 Sentence denotes Despite this high homology, PP4 does not appear to associate with known PP2A regulatory subunits.
T4 364-532 Sentence denotes As a first step toward characterization of PP4 holoenzymes and identification of putative PP4 regulatory subunits, PP4 was purified from bovine testis soluble extracts.
T5 533-650 Sentence denotes PP4 existed in two complexes of approximately 270-300 and 400-450 kDa as determined by gel filtration chromatography.
T6 651-788 Sentence denotes The smaller PP4 complex was purified by sequential phenyl-Sepharose, Source 15Q, DEAE2, and Superdex 200 gel filtration chromatographies.
T7 789-962 Sentence denotes The final product contained two major proteins: the PP4 catalytic subunit plus a protein that migrated as a doublet of 120-125 kDa on SDS-polyacrylamide gel electrophoresis.
T8 963-1046 Sentence denotes The associated protein, termed PP4R1, and PP4C also bound to microcystin-Sepharose.
T9 1047-1157 Sentence denotes Mass spectrometry analysis of the purified complex revealed two major peaks, at 35 (PP4C) and 105 kDa (PP4R1).
T10 1158-1286 Sentence denotes Amino acid sequence information of several peptides derived from the 105 kDa protein was utilized to isolate a human cDNA clone.
T11 1287-1424 Sentence denotes Analysis of the predicted amino acid sequence revealed 13 nonidentical repeats similar to repeats found in the A subunit of PP2A (PP2AA).
T12 1425-1567 Sentence denotes The PP4R1 cDNA clone engineered with an N-terminal Myc tag was expressed in COS M6 cells and PP4C co-immunoprecipitated with Myc-tagged PP4R1.
T13 1568-1729 Sentence denotes These data indicate that one form of PP4 is similar to the core complex of PP2A in that it consists of a catalytic subunit and a "PP2AA-like" structural subunit.